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Literature summary for 1.1.1.37 extracted from

  • Hartl, T.; Grossebueter, W.; Goerisch, H.; Stezowski, J.J.
    Crystalline NAD/NADP-dependent malate dehydrogenase; the enzyme from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius (1987), Biol. Chem. Hoppe-Seyler, 368, 259-267.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sulfolobus acidocaldarius
in presence of ethylene glycol 4000 Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
(S)-malate product inhibition, 66% inhibition at 5 mM Sulfolobus acidocaldarius
2-oxoglutarate 44% inhibition at 5 mM Sulfolobus acidocaldarius
alpha-ketoglutarate
-
Methanospirillum hungatei
alpha-ketoglutarate
-
Sulfolobus acidocaldarius
L-malate
-
Methanospirillum hungatei
L-malate product inhibition Sulfolobus acidocaldarius
L-malate
-
Sus scrofa
additional information not inhibitory: AMP, ADP, ATP Sulfolobus acidocaldarius
NAD+ product inhibition; product inhibition, 29% inhibition at 5 mM, 42% inhibition at 10 mM Sulfolobus acidocaldarius
NADP+ product inhibition; product inhibition, 26% inhibition at 10 mM Sulfolobus acidocaldarius
oxaloacetate substrate inhibition; substrate inhibition, 29% inhibition at 5 mM Sulfolobus acidocaldarius
oxaloacetate
-
Sus scrofa
oxaloacetate substrate inhibition Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
NADH
-
Sulfolobus acidocaldarius
0.0041
-
NADH 40°C, pH 5.8 Sulfolobus acidocaldarius
0.052
-
oxaloacetate
-
Sulfolobus acidocaldarius
0.052
-
oxaloacetate 40°C, pH 5.8 Sulfolobus acidocaldarius
0.2
-
NADPH
-
Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
4 * 34000, SDS-PAGE Sulfolobus acidocaldarius
97000
-
gel filtration Sulfolobus acidocaldarius
128500
-
ultracentrifugation Sulfolobus acidocaldarius
128500
-
ultracentrifugation, sedimentation equilibrium studies Sulfolobus acidocaldarius
134000
-
cross-linking data Sulfolobus acidocaldarius
134000
-
SDS-PAGE of enzyme after cross-linking Sulfolobus acidocaldarius
140000
-
-
Laceyella sacchari

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Thermus aquaticus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Thermus thermophilus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Sus scrofa
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Methanothermobacter thermautotrophicus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Sulfolobus acidocaldarius
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Thermoplasma acidophilum
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Laceyella sacchari
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Laceyella sacchari
-
-
-
Methanospirillum hungatei
-
-
-
Methanothermobacter thermautotrophicus
-
-
-
Sulfolobus acidocaldarius
-
-
-
Sus scrofa
-
pig
-
Thermoplasma acidophilum
-
-
-
Thermus aquaticus
-
-
-
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
L-malate oxidation in presence of NAD+ Sulfolobus acidocaldarius
277
-
-
Sulfolobus acidocaldarius
277
-
40°C, pH 5.8 Sulfolobus acidocaldarius

Storage Stability

Storage Stability Organism
-20°C, can be stored over months Sulfolobus acidocaldarius
4°C, pH 7.0-9.0, concentration of 0.0018 mg/ml, quite stable. Below pH 7.0, inactivation occurs Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Thermus aquaticus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Thermus thermophilus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Sus scrofa oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Sulfolobus acidocaldarius oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Thermoplasma acidophilum oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Laceyella sacchari oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ low activity in this direction Sulfolobus acidocaldarius oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Thermus aquaticus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Thermus thermophilus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Sus scrofa oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Sulfolobus acidocaldarius oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Thermoplasma acidophilum oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Laceyella sacchari oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH
-
Thermus aquaticus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Thermus thermophilus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Escherichia coli L-malate + NAD+
-
r
oxaloacetate + NADH
-
Sus scrofa L-malate + NAD+
-
r
oxaloacetate + NADH
-
Methanothermobacter thermautotrophicus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Sulfolobus acidocaldarius L-malate + NAD+
-
r
oxaloacetate + NADH
-
Thermoplasma acidophilum L-malate + NAD+
-
r
oxaloacetate + NADH
-
Methanospirillum hungatei L-malate + NAD+
-
?
oxaloacetate + NADH
-
Laceyella sacchari L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Sulfolobus acidocaldarius (S)-malate + NAD+
-
r
oxaloacetate + NADPH + H+
-
Sulfolobus acidocaldarius (S)-malate + NADP+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Escherichia coli
tetramer
-
Thermoplasma acidophilum
tetramer
-
Laceyella sacchari
tetramer 4 * 34000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
8 80
-
Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
activity of purified enzyme decreases continually from 4°C to 80°C Sulfolobus acidocaldarius
75
-
15 min, 75% residual activity, purified enzyme Sulfolobus acidocaldarius
80
-
15 min, no loss of activity, enzyme in crude extract Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.62
-
oxaloacetate
-
Sulfolobus acidocaldarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
complete inactivation Sulfolobus acidocaldarius
5.5
-
24 h, 50% loss of activity Sulfolobus acidocaldarius
7
-
inactivation below Sulfolobus acidocaldarius
7 9 quite stable Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sulfolobus acidocaldarius
NAD+
-
Thermoplasma acidophilum
NADH A-side stereospecificity Sulfolobus acidocaldarius
NADP+
-
Sulfolobus acidocaldarius
NADP+
-
Thermoplasma acidophilum
NADPH A-side stereospecificity Sulfolobus acidocaldarius