Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Picrophilus torridus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 20% (v/v), 40% loss of activity | Picrophilus torridus | |
ATP | inhibition displays Michaelis-Menten kinetics in a noncompetitive mode with respect to the cofactor NADP+ | Picrophilus torridus | |
ethanol | 20% (v/v), 20% loss of activity | Picrophilus torridus | |
methanol | 20% (v/v), 30% loss of activity | Picrophilus torridus | |
additional information | EDTA added at up to 10 mm causes no loss of activity. Pyruvate, phosphoenolpyruvate, 3-phosphoglycerate, 2-phosphoglycerate, phosphate and diphosphate do not affect the activity when added to the standard assay at 5 or 20 mm | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NAD+ | the precise determination of the Km for NAD+ is not possible, as it is impossible to reach saturation of the enzyme | Picrophilus torridus | |
1.12 | - |
NADP+ | pH 6.5, 55°C, cosubstrate: D-glucose | Picrophilus torridus | |
4.5 | - |
D-galactose | pH 6.5, 55°C, cosubstrate: NADP+ | Picrophilus torridus | |
10 | - |
D-glucose | pH 6.5, 55°C, cosubstrate: NADP+ | Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of 5 mM NaCl, MgCl2, MnCl2 or CaCl2 has no effect | Picrophilus torridus | |
Zn2+ | the enzyme contains structurally important zinc, the enzyme also contains Zn2+ near the catalytic site. Addition of ZnCl2 to the assay buffer at up to 5 mM has no effect on activity | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40462 | - |
4 * 40462, calculated from sequence | Picrophilus torridus |
40500 | - |
4 * 40500, SDS-PAGE | Picrophilus torridus |
160000 | - |
gel filtration | Picrophilus torridus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 50% (v/v), no detectable loss of activity | Picrophilus torridus |
Ethanol | 50% (v/v), no detectable loss of activity | Picrophilus torridus |
Methanol | 50% (v/v), no detectable loss of activity | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6L047 | - |
- |
Picrophilus torridus DSM 9790 | Q6L047 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-galactopyranose + NADP+ = D-galactono-1,5-lactone + NADPH + H+ | (2) | Picrophilus torridus | |
D-glucopyranose + NADP+ = D-glucono-1,5-lactone + NADPH + H+ | (1) | Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
252 | - |
pH 6.5, 55°C, NADP+ (cosubstrate: D-glucose) | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ + H2O | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus | D-galactonate + NADH + 2 H+ | - |
? | |
D-galactose + NAD+ + H2O | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus DSM 9790 | D-galactonate + NADH + 2 H+ | - |
? | |
D-galactose + NADP+ + H2O | 74% of the activity with D-glucose + NADP+ | Picrophilus torridus | D-galactonate + NADPH + 2 H+ | - |
? | |
D-galactose + NADP+ + H2O | 74% of the activity with D-glucose + NADP+ | Picrophilus torridus DSM 9790 | D-galactonate + NADPH + 2 H+ | - |
? | |
D-glucose + NAD+ + H2O | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus | D-gluconate + NADH + 2 H+ | - |
? | |
D-glucose + NAD+ + H2O | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus DSM 9790 | D-gluconate + NADH + 2 H+ | - |
? | |
D-glucose + NADP+ + H2O | - |
Picrophilus torridus | D-gluconate + NADPH + 2 H+ | - |
? | |
D-glucose + NADP+ + H2O | - |
Picrophilus torridus DSM 9790 | D-gluconate + NADPH + 2 H+ | - |
? | |
additional information | none of the C2 and C3 epimers of D-glucose or derivatives (D-mannose, D-allose, D-glucosamine, 2-deoxy-D-glucose, glucose-6-phosphate) and none of the aldopentoses (D-xylose, L-arabinose, D-ribose) tested shows activity above 2% both with NADP+ and NAD+ as cosubstrates | Picrophilus torridus | ? | - |
? | |
additional information | none of the C2 and C3 epimers of D-glucose or derivatives (D-mannose, D-allose, D-glucosamine, 2-deoxy-D-glucose, glucose-6-phosphate) and none of the aldopentoses (D-xylose, L-arabinose, D-ribose) tested shows activity above 2% both with NADP+ and NAD+ as cosubstrates | Picrophilus torridus DSM 9790 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40462, calculated from sequence | Picrophilus torridus |
tetramer | 4 * 40500, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
dual-specific glucose/galactose dehydrogenase | - |
Picrophilus torridus |
GdhA | - |
Picrophilus torridus |
glucose (galactose) dehydrogenase | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
substrates: glucose + NADP+ | Picrophilus torridus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | 55°C: optimum, 60°C: 88% of maximal activity | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Zn2+ improves thermal stability. EDTA completely abolishes the stabilizing effect of Zn2+ | Picrophilus torridus |
65 | - |
pH 6.5, without the addition of Zn2+, t1/2: 3 h | Picrophilus torridus |
70 | - |
pH 6.5, without the addition of Zn2+, t1/2: 5 min. At 1 mM Zn2+ the enzyme is stable for 3 h | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Picrophilus torridus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus | |
NADP+ | prefers NADP+ over NAD+. Approximately 20-fold higher activity with NADP+ | Picrophilus torridus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
ATP | pH 6.5, 55°C, at saturating glucose concentration (50 mM) | Picrophilus torridus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in glucose catabolism via a nonphosphorylated variant of the EntnerDoudoroff pathway | Picrophilus torridus |