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Literature summary for 1.1.1.36 extracted from

  • Qin, Y.M.; Marttila, M.S.; Haapalainen, A.M.; Siivari, K.M.; Glumoff, T.; Hiltunen, J.K.
    Yeast peroxisomal multifunctional enzyme: (3R)-hydroxyacyl-CoA dehydrogenase domains A and B are required for optimal growth on oleic acid (1999), J. Biol. Chem., 274, 28619-28625.
    View publication on PubMed
Search for more literature for 1.1.1.36

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the yeast expression plasmid pYE352, Saccharomyces cerevisiae fox-2 cells, devoid of Sc MFE-2, transformed with pYE352::ScMFE-2(adelta) and pYE352::ScMFE-2(bdelta). The recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] transformed into Escherichia coli Candida tropicalis

Protein Variants

Protein Variants Comment Organism
A+/B+CtMFE-2(h2delta) recombinant enzyme Candida tropicalis
A-/B+CtMFE-2(hdelta2deltaadelta) domain A deleted Candida tropicalis
A-/B-CtMFE-2(hdelta2deltabdelta) domain A and B deleted Candida tropicalis
G16S site-directed mutagenesis Saccharomyces cerevisiae
G329S site-directed mutagenesis Saccharomyces cerevisiae
additional information the Candida tropicalis MFE-2 with a deleted hydratase 2 domain (Ct MFE-2(h2delta)) and mutational variants of the A and B domains (Ct MFE-2(h2deltaadelta), Ct MFE-2(h2deltabdelta), and Ct MFE-2(h2deltaadeltabdelta)) are overexpressed and characterized Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 (3R)-3-hydroxydecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
0.0054
-
 (3R)-3-hydroxydecanoyl-CoA A-/B-CtMFE-2(hdelta2deltabdelta) Candida tropicalis
0.0058
-
 (3R)-3-hydroxydecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
0.024
-
 (3R)-hydroxyhexadecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
0.024
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B-CtMFE-2(hdelta2deltabdelta) Candida tropicalis
0.025
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B+CtMFE-2(hdelta2deltabdelta) Candida tropicalis
0.055
-
 (3R)-hydroxybutyryl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
0.055
-
 (3R)-hydroxybutyryl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Homo sapiens 5777
-
peroxisome
-
 Saccharomyces cerevisiae 5777
-
peroxisome
-
 Candida tropicalis 5777
-

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-
Homo sapiens
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-
Saccharomyces cerevisiae
-
 alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown
-

Purification (Commentary)

Purification (Comment) Organism
Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Hs MFE-2 Homo sapiens
0.5
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc A-/B+ Saccharomyces cerevisiae
0.9
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc B-/A+ Saccharomyces cerevisiae
1.3
-
 (3R)-hydroxyacyl-CoA dehydrogenase, Sc MFE-2 Saccharomyces cerevisiae
125
-
 last purification step, 5.9fold purification. Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-hydroxydecanoyl-CoA + NADP+ (3R)-hydroxyacyl-CoA dehydrogenase Candida tropicalis 3-oxodecanoyl-CoA + NADPH + H+
-
 ?
(3R)-hydroxybutyryl-CoA + NADP+
-
 Candida tropicalis 3-oxobutyryl-CoA + NADPH + H+
-
 ?
(3R)-hydroxyhexadecanoyl-CoA + NADP+
-
 Candida tropicalis 3-oxohexadecanoyl-CoA + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
(3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Homo sapiens
(3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Saccharomyces cerevisiae
(3R)-hydroxyacyl-CoA dehydrogenase domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 Candida tropicalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 (3R)-hydroxyhexadecanoyl-CoA A-/B-CtMFE-2(hdelta2delta) Candida tropicalis
12
-
 (3R)-hydroxyhexadecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
17
-
 (3R)-3-hydroxydecanoyl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
29
-
 (3R)-hydroxybutyryl-CoA A-/B+CtMFE-2(hdelta2deltaadelta) Candida tropicalis
31
-
 (3R)-hydroxybutyryl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
33
-
 (3R)-3-hydroxydecanoyl-CoA A-/B-CtMFE-2(hdelta2delta) Candida tropicalis
41
-
 (3R)-hydroxyhexadecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis
53
-
 (3R)-3-hydroxydecanoyl-CoA A+/B+CtMFE-2(h2delta) Candida tropicalis