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Literature summary for 1.1.1.36 extracted from

  • Satoh, Y.; Tajima, K.; Tannai, H.; Munekata, M.
    Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro (2003), J. Biosci. Bioeng., 95, 335-341.
    View publication on PubMed
Search for more literature for 1.1.1.36

Application

Application Comment Organism
synthesis establishing of an enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro on the basis of the poly(3-hydroxybutyrate) biosynthesis pathway of Ralstonia eutropha, recycling CoA for synthesis of acetyl-CoA and deriving NADPH from regeneration by GDH, overview Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
gene phaB, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
NADP+ competitive inhibition Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+ step in the enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro, overview Cupriavidus necator (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
NADPH-dependent acetoacetyl-CoA reductase
-
 Cupriavidus necator
PhaB
-
 Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 assay at Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Cupriavidus necator