KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Fusidic acid | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.0068 | - |
Fusidic acid | pH 8.9, temperature not specified in the publication, NAD(P)+ | Pseudomonas sp. | |
0.0111 | - |
5beta-dihydrotestosterone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.0152 | - |
3alpha-hydroxydesogestrel | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.0161 | - |
5alpha-dihydrotestosterone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.019 | - |
5alpha-dihydrotestosterone | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
0.0201 | - |
5beta-dihydrotestosterone | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
0.0207 | - |
epiandrosterone | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.0216 | - |
3alpha-hydroxydesogestrel | pH 8.9, temperature not specified in the publication, NAD(P)+ | Pseudomonas sp. | |
0.0244 | - |
androsterone | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.025 | - |
3-dehydro-alpha-ecdysone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.025 | - |
cholic acid | pH 8.9, temperature not specified in the publication, NAD(P)+ | Pseudomonas sp. | |
0.0267 | - |
androsterone | pH 8.9, temperature not specified in the publication, NAD(P)+ | Pseudomonas sp. | |
0.028 | - |
3-dehydro-alpha-ecdysone | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
0.0301 | - |
cholic acid | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.0328 | - |
androstandiol | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni | |
0.035 | - |
3-dehydro-beta-ecdysone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.0357 | - |
androstandione | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.61 | - |
Metyrapone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
0.63 | - |
Metyrapone | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
0.9 | - |
4-Nitroacetophenone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
1 | - |
4-nitrobenzaldehyde | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
1 | - |
4-Nitroacetophenone | pH 7.0, temperature not specified in the publication, NAD(P)H | Pseudomonas sp. | |
1 | - |
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
1.09 | - |
4-nitrobenzaldehyde | pH 7.0, temperature not specified in the publication, NAD(P)H | Comamonas testosteroni | |
2.1 | - |
metyrapol | pH 8.9, temperature not specified in the publication, NAD(P)+ | Comamonas testosteroni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Comamonas testosteroni | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Comamonas testosteroni |
28000 | - |
SDS-PAGE | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ | Comamonas testosteroni | - |
a 3-oxosteroid + NAD(P)H + H+ | - |
? | |
a 3alpha-hydroxysteroid + NAD(P)+ | Pseudomonas sp. | - |
a 3-oxosteroid + NAD(P)H + H+ | - |
? | |
a 3alpha-hydroxysteroid + NAD(P)+ | Comamonas testosteroni ATCC 11996 | - |
a 3-oxosteroid + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | P80702 | - |
- |
Comamonas testosteroni ATCC 11996 | P80702 | - |
- |
Pseudomonas sp. | P80701 | Boehringer patent strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ | 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 | Comamonas testosteroni | 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+ | - |
? | |
2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ | 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 | Comamonas testosteroni ATCC 11996 | 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+ | - |
? | |
3-dehydro-alpha-ecdysone + NAD(P)H + H+ | - |
Comamonas testosteroni | alpha-ecdysone + NAD(P)+ | - |
? | |
3-dehydro-alpha-ecdysone + NAD(P)H + H+ | - |
Pseudomonas sp. | alpha-ecdysone + NAD(P)+ | - |
? | |
3-dehydro-alpha-ecdysone + NAD(P)H + H+ | - |
Comamonas testosteroni ATCC 11996 | alpha-ecdysone + NAD(P)+ | - |
? | |
3-dehydro-beta-ecdysone + NAD(P)H + H+ | - |
Comamonas testosteroni | beta-ecdysone + NAD(P)+ | - |
? | |
3-dehydro-beta-ecdysone + NAD(P)H + H+ | - |
Comamonas testosteroni ATCC 11996 | beta-ecdysone + NAD(P)+ | - |
? | |
3alpha-hydroxydesogestrel + NAD(P)+ | - |
Comamonas testosteroni | 3-oxodesogestrel + NAD(P)H + H+ | - |
? | |
3alpha-hydroxydesogestrel + NAD(P)+ | - |
Pseudomonas sp. | 3-oxodesogestrel + NAD(P)H + H+ | - |
? | |
4-nitroacetophenone + NAD(P)H + H+ | - |
Comamonas testosteroni | 1-(4-nitrophenyl)ethanol + NAD(P)+ | - |
? | |
4-nitroacetophenone + NAD(P)H + H+ | - |
Pseudomonas sp. | 1-(4-nitrophenyl)ethanol + NAD(P)+ | - |
? | |
4-nitrobenzaldehyde + NAD(P)H + H+ | - |
Comamonas testosteroni | (4-nitrophenyl)methanol + NAD(P)+ | - |
? | |
4-nitrobenzaldehyde + NAD(P)H + H+ | - |
Pseudomonas sp. | (4-nitrophenyl)methanol + NAD(P)+ | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | (5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Pseudomonas sp. | (5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
? | |
5beta-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | (5beta,17beta)-androstane-3,17-diol + NAD(P)+ | - |
? | |
5beta-dihydrotestosterone + NAD(P)H + H+ | - |
Pseudomonas sp. | (5beta,17beta)-androstane-3,17-diol + NAD(P)+ | - |
? | |
a 3alpha-hydroxysteroid + NAD(P)+ | - |
Comamonas testosteroni | a 3-oxosteroid + NAD(P)H + H+ | - |
? | |
a 3alpha-hydroxysteroid + NAD(P)+ | - |
Pseudomonas sp. | a 3-oxosteroid + NAD(P)H + H+ | - |
? | |
a 3alpha-hydroxysteroid + NAD(P)+ | - |
Comamonas testosteroni ATCC 11996 | a 3-oxosteroid + NAD(P)H + H+ | - |
? | |
androstandiol + NAD(P)+ | - |
Comamonas testosteroni | (5alpha,17beta)-17-hydroxyandrostan-3-one + NAD(P)H + H+ | - |
? | |
androstandione + NAD(P)H + H+ | - |
Comamonas testosteroni | 3-hydroxyandrostan-17-one + NAD(P)+ | - |
? | |
androsterone + NAD(P)+ | - |
Comamonas testosteroni | (5alpha)-androstane-3,17-dione + NAD(P)H + H+ | - |
? | |
androsterone + NAD(P)+ | - |
Pseudomonas sp. | (5alpha)-androstane-3,17-dione + NAD(P)H + H+ | - |
? | |
cholic acid + NAD(P)+ | - |
Comamonas testosteroni | (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+ | - |
? | |
cholic acid + NAD(P)+ | - |
Pseudomonas sp. | (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+ | - |
? | |
epiandrosterone + NAD(P)+ | - |
Comamonas testosteroni | (5alpha)-androstane-3,17-dione + NAD(P)H + H+ | - |
? | |
fusidic acid + NAD(P)+ | - |
Comamonas testosteroni | (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+ | - |
? | |
fusidic acid + NAD(P)+ | - |
Pseudomonas sp. | (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+ | - |
? | |
metyrapol + NAD(P)+ | - |
Comamonas testosteroni | 2-methyl-1,2-di(pyridin-3-yl)propan-1-one + NAD(P)H + H+ | - |
? | |
metyrapone + NAD(P)H + H+ | - |
Comamonas testosteroni | 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+ | - |
? | |
metyrapone + NAD(P)H + H+ | - |
Pseudomonas sp. | 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+ | - |
? | |
additional information | no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol | Comamonas testosteroni | ? | - |
? | |
additional information | no activity with testosterone, 3-oxo-desogestrel, progesterone, NKI 42255, trans-benzene dihydrodiol and cis-benzene dihydrodiol | Pseudomonas sp. | ? | - |
? | |
additional information | no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol | Comamonas testosteroni ATCC 11996 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Comamonas testosteroni |
monomer | 1 * 28000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
HSD 28 | - |
Comamonas testosteroni |
HSD 29 | - |
Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is involved in inactivation of steroid hormones | Comamonas testosteroni |
metabolism | enzyme is involved in inactivation of steroid hormones | Pseudomonas sp. |