Cloned (Comment) | Organism |
---|---|
- |
Yersinia pseudotuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose | pH 7.5, 37°C, cosubstrate: NADPH | Yersinia pseudotuberculosis | |
0.139 | - |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose | pH 7.5, 37°C, cosubstrate: NADH | Yersinia pseudotuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35234 | - |
2 * 35234, calculated from sequence | Yersinia pseudotuberculosis |
36000 | - |
2 * 36000, SDS-PAGE | Yersinia pseudotuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ | Yersinia pseudotuberculosis | the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides | GDP-beta-L-colitose + NAD(P)+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ | Yersinia pseudotuberculosis VIA | the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides | GDP-beta-L-colitose + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pseudotuberculosis | - |
- |
- |
Yersinia pseudotuberculosis VIA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
N-terminal His6-tagged ColC | Yersinia pseudotuberculosis |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Yersinia pseudotuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ | the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides | Yersinia pseudotuberculosis | GDP-beta-L-colitose + NAD(P)+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ | the enzyme is involved in GDP-L-fucose biosynthesis. L-Colitose is a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides | Yersinia pseudotuberculosis VIA | GDP-beta-L-colitose + NAD(P)+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+ | bifunctional enzyme catalyzing the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose | Yersinia pseudotuberculosis | GDP-beta-L-colitose + NAD+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+ | bifunctional enzyme catalyzing the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose | Yersinia pseudotuberculosis VIA | GDP-beta-L-colitose + NAD+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+ | the enzyme catalyzes the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose. The stereochemical preference of ColC with regard to the transfer of one of the diastereotopic methylene hydrogens at C-4 of the dihydronicotinamide ring is established to be pro-S stereospecific | Yersinia pseudotuberculosis | GDP-beta-L-colitose + NADP+ | - |
? | |
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+ | the enzyme catalyzes the C-5 epimerization of GDP-4-dehydro-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose. The stereochemical preference of ColC with regard to the transfer of one of the diastereotopic methylene hydrogens at C-4 of the dihydronicotinamide ring is established to be pro-S stereospecific | Yersinia pseudotuberculosis VIA | GDP-beta-L-colitose + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Yersinia pseudotuberculosis |
homodimer | 2 * 35234, calculated from sequence | Yersinia pseudotuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ColC | - |
Yersinia pseudotuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Yersinia pseudotuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Yersinia pseudotuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Yersinia pseudotuberculosis | |
NADPH | - |
Yersinia pseudotuberculosis |