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Literature summary for 1.1.1.35 extracted from

  • Winkler, U.; Saftel, W.; Stabenau, H.
    A new type of a multifunctional beta-oxidation enzyme in Euglena (2003), Plant Physiol., 131, 753-762.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
acetoacetyl-CoA pH 6.5 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Euglena gracilis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE Euglena gracilis
34000
-
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE Euglena gracilis
44500
-
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE Euglena gracilis
45500
-
1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE Euglena gracilis
460000
-
native enzyme complex, sucrose density gradient centrifugation Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Euglena gracilis both enzyme activities are specific for L-isomers acetoacetyl-CoA + NADH
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ Euglena gracilis 1224-5/25 both enzyme activities are specific for L-isomers acetoacetyl-CoA + NADH
-
r

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
multifunctional beta-oxidation enzyme complex composed of 2 different 3-hydroyacyl-CoA dehydrogenases, 2 different 2-enoyl-CoA hydratases, thiolase, and epimerase activities
-
Euglena gracilis 1224-5/25
-
multifunctional beta-oxidation enzyme complex composed of 2 different 3-hydroyacyl-CoA dehydrogenases, 2 different 2-enoyl-CoA hydratases, thiolase, and epimerase activities
-

Purification (Commentary)

Purification (Comment) Organism
beta-oxidation enzyme complex 67fold to homogeneity by ammonium sulfate precipitation, density gradient centrifugation, and ion-exchange chromatography Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
purified enzyme Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ both enzyme activities are specific for L-isomers Euglena gracilis acetoacetyl-CoA + NADH
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ both enzyme activities are specific for L-isomers Euglena gracilis 1224-5/25 acetoacetyl-CoA + NADH
-
r
acetoacetyl-CoA + NADH + H+
-
Euglena gracilis (S)-3-hydroxybutyryl-CoA + NAD+ both enzyme activities are specific for L-isomers r
acetoacetyl-CoA + NADH + H+
-
Euglena gracilis 1224-5/25 (S)-3-hydroxybutyryl-CoA + NAD+ both enzyme activities are specific for L-isomers r

Subunits

Subunits Comment Organism
More the hydratase and the thiolase functions are located on the large subunit, whereas 2 dehydrogenase functions are located on the 2 smaller subunits, epimerase activity is only measurable in the complete enzyme complex Euglena gracilis
tetramer 1 * 45500 + 1 * 44500 + 1 * 34000 + 1 * 32000, SDS-PAGE Euglena gracilis

Synonyms

Synonyms Comment Organism
3-hydroxyacyl-coenzyme A dehydrogenase
-
Euglena gracilis
multifunctional beta-oxidation enzyme
-
Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Euglena gracilis