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Literature summary for 1.1.1.348 extracted from

  • Guo, L.; Paiva, N.L.
    Molecular cloning and expression of alfalfa (Medicago sativa L.) vestitone reductase, the penultimate enzyme in medicarpin biosynthesis (1995), Arch. Biochem. Biophys., 320, 353-360.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Medicago sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35918
-
x * 35918, calculated from amino acid sequence Medicago sativa
38000
-
x * 38000, SDS-PAGE Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-vestitone + NADPH + H+ Medicago sativa
-
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information not detected in stem, petiole and leaf Medicago sativa
-
root the highest levels of transcript is in the root Medicago sativa
-
root nodule relatively high transcript levels are found in the nodule samples Medicago sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-vestitone + NADPH + H+
-
Medicago sativa (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Medicago sativa
? x * 35918, calculated from amino acid sequence Medicago sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Medicago sativa

Expression

Organism Comment Expression
Medicago sativa the levels of vestitone reductase transcript greatly increase within 2 h of elicitor addition to alfalfa cell suspension cultures up

General Information

General Information Comment Organism
metabolism vestitone reductase is the penultimate enzyme in medicarpin biosynthesis Medicago sativa