Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 Star(DE3) | Castellaniella defragrans |
expression in Escherichia coli | Castellaniella defragrans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide | I80 of less than 34 mM | Castellaniella defragrans | |
diethylpyrocarbonate | I80 of less than 17 mM | Castellaniella defragrans | |
EDTA | 7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96% | Castellaniella defragrans | |
N-ethylmaleimide | I80 of less than 0.5 mM | Castellaniella defragrans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
geraniol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.0044 | - |
(S)-perillyl alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.005 | - |
geraniol | pH 9.4, 21°C | Castellaniella defragrans | |
0.006 | - |
cumic alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.007 | - |
(S)-perillyl alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.007 | - |
(S)-perillyl alcohol | pH 9.4, 21°C | Castellaniella defragrans | |
0.021 | - |
cumic alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.021 | - |
p-cumic alcohol | pH 9.4, 21°C | Castellaniella defragrans | |
0.0232 | - |
nerol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.045 | - |
nerol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.045 | - |
nerol | pH 9.4, 21°C | Castellaniella defragrans | |
0.05 | - |
geraniol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.0575 | - |
citronellol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.086 | - |
citronellol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.086 | - |
citronellol | pH 9.4, 21°C | Castellaniella defragrans | |
0.1157 | - |
benzyl alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.17 | - |
benzyl alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
0.17 | - |
benzyl alcohol | pH 9.4, 21°C | Castellaniella defragrans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Castellaniella defragrans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38272 | - |
2 * 38272, calculated from amino acid sequence | Castellaniella defragrans |
38272 | - |
2 * 38272, calculated | Castellaniella defragrans |
39000 | - |
2 * 39000, SDS-PAGE | Castellaniella defragrans |
52000 | - |
gel filtration | Castellaniella defragrans |
52000 | 91000 | gel filtration | Castellaniella defragrans |
85000 | - |
PAGE | Castellaniella defragrans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + NAD+ | Castellaniella defragrans | - |
geranial + NADH + H+ | - |
? | |
geraniol + NAD+ | Castellaniella defragrans 65Phen | - |
geranial + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Castellaniella defragrans | H1ZV38 | - |
- |
Castellaniella defragrans 65Phen | H1ZV38 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography and phenyl-Sepharose 6 gel filtration | Castellaniella defragrans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21°C | Castellaniella defragrans |
4.33 | - |
pH 9.4, 21°C | Castellaniella defragrans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-perillyl alcohol + NAD+ | most efficient substrate | Castellaniella defragrans | ? + NADH + H+ | - |
? | |
(S)-perillyl alcohol + NAD+ | - |
Castellaniella defragrans | perillyl aldehyde + NADH | - |
? | |
benzyl alcohol + NAD+ | - |
Castellaniella defragrans | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Castellaniella defragrans 65Phen | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Castellaniella defragrans | ? | - |
? | |
citronellol + NAD+ | - |
Castellaniella defragrans | ? | - |
? | |
citronellol + NAD+ | - |
Castellaniella defragrans | citronellal + NADH + H+ | - |
? | |
cumic alcohol + NAD+ | - |
Castellaniella defragrans | ? | - |
? | |
geraniol + dichlorophenolindophenol | - |
Castellaniella defragrans | ? | - |
? | |
geraniol + NAD+ | - |
Castellaniella defragrans | geranial + NADH + H+ | - |
? | |
geraniol + NAD+ | - |
Castellaniella defragrans 65Phen | geranial + NADH + H+ | - |
? | |
geraniol + oxidized dichlorophenolindophenol | - |
Castellaniella defragrans | geranial + reduced dichlorophenolindophenol | - |
? | |
additional information | the enzyme cannot use phenazine methosulfate as alternative acceptor | Castellaniella defragrans | ? | - |
? | |
additional information | phenazine methosulfate is not accepted as an electron acceptor | Castellaniella defragrans | ? | - |
? | |
additional information | the enzyme cannot use phenazine methosulfate as alternative acceptor | Castellaniella defragrans 65Phen | ? | - |
? | |
additional information | phenazine methosulfate is not accepted as an electron acceptor | Castellaniella defragrans 65Phen | ? | - |
? | |
nerol + NAD+ | - |
Castellaniella defragrans | neral + NADH + H+ | - |
? | |
nerol + NAD+ | - |
Castellaniella defragrans 65Phen | neral + NADH + H+ | - |
? | |
nerol + NAD+ | - |
Castellaniella defragrans | ? | - |
? | |
p-cumic alcohol + NAD+ | - |
Castellaniella defragrans | ? + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38272, calculated | Castellaniella defragrans |
homodimer | 2 * 39000, SDS-PAGE | Castellaniella defragrans |
homodimer | 2 * 38272, calculated from amino acid sequence | Castellaniella defragrans |
Synonyms | Comment | Organism |
---|---|---|
GeDH | - |
Castellaniella defragrans |
geoA | - |
Castellaniella defragrans |
geraniol DH | - |
Castellaniella defragrans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
- |
Castellaniella defragrans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH is ineffective as a cosubstrate | Castellaniella defragrans | |
NAD+ | dependent on, no activity with NADP+ | Castellaniella defragrans | |
NADH | enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided | Castellaniella defragrans |
Organism | Comment | Expression |
---|---|---|
Castellaniella defragrans | enzyme activity is induced during growth with limonene in comparison to growth with acetate | up |
Castellaniella defragrans | activitiy is induced during growth with limonene in comparison to growth with acetate | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
citronellol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
100 | - |
citronellol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
100 | - |
citronellol | pH 9.4, 21°C | Castellaniella defragrans | |
110 | - |
benzyl alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
220 | - |
benzyl alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
220 | - |
benzyl alcohol | pH 9.4, 21°C | Castellaniella defragrans | |
310 | - |
nerol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
310 | - |
nerol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
310 | - |
nerol | pH 9.4, 21°C | Castellaniella defragrans | |
520 | - |
cumic alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
520 | - |
p-cumic alcohol | pH 9.4, 21°C | Castellaniella defragrans | |
620 | - |
geraniol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
940 | - |
cumic alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
1570 | - |
geraniol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
1570 | - |
geraniol | pH 9.4, 21°C | Castellaniella defragrans | |
2020 | - |
(S)-perillyl alcohol | native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans | |
2020 | - |
(S)-perillyl alcohol | pH 9.4, 21°C | Castellaniella defragrans | |
3550 | - |
(S)-perillyl alcohol | recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C | Castellaniella defragrans |