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Literature summary for 1.1.1.347 extracted from

  • Lueddeke, F.; Wuelfing, A.; Timke, M.; Germer, F.; Weber, J.; Dikfidan, A.; Rahnfeld, T.; Linder, D.; Meyerdierks, A.; Harder, J.
    Geraniol and geranial dehydrogenases induced in anaerobic monoterpene degradation by Castellaniella defragrans (2012), Appl. Environ. Microbiol., 78, 2128-2136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Star(DE3) Castellaniella defragrans
expression in Escherichia coli Castellaniella defragrans

Inhibitors

Inhibitors Comment Organism Structure
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide I80 of less than 34 mM Castellaniella defragrans
diethylpyrocarbonate I80 of less than 17 mM Castellaniella defragrans
EDTA 7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96% Castellaniella defragrans
N-ethylmaleimide I80 of less than 0.5 mM Castellaniella defragrans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
geraniol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.0044
-
(S)-perillyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.005
-
geraniol pH 9.4, 21°C Castellaniella defragrans
0.006
-
cumic alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.007
-
(S)-perillyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.007
-
(S)-perillyl alcohol pH 9.4, 21°C Castellaniella defragrans
0.021
-
cumic alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.021
-
p-cumic alcohol pH 9.4, 21°C Castellaniella defragrans
0.0232
-
nerol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.045
-
nerol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.045
-
nerol pH 9.4, 21°C Castellaniella defragrans
0.05
-
geraniol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.0575
-
citronellol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.086
-
citronellol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.086
-
citronellol pH 9.4, 21°C Castellaniella defragrans
0.1157
-
benzyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.17
-
benzyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
0.17
-
benzyl alcohol pH 9.4, 21°C Castellaniella defragrans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Castellaniella defragrans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38272
-
2 * 38272, calculated from amino acid sequence Castellaniella defragrans
38272
-
2 * 38272, calculated Castellaniella defragrans
39000
-
2 * 39000, SDS-PAGE Castellaniella defragrans
52000
-
gel filtration Castellaniella defragrans
52000 91000 gel filtration Castellaniella defragrans
85000
-
PAGE Castellaniella defragrans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + NAD+ Castellaniella defragrans
-
geranial + NADH + H+
-
?
geraniol + NAD+ Castellaniella defragrans 65Phen
-
geranial + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Castellaniella defragrans H1ZV38
-
-
Castellaniella defragrans 65Phen H1ZV38
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography and phenyl-Sepharose 6 gel filtration Castellaniella defragrans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21°C Castellaniella defragrans
4.33
-
pH 9.4, 21°C Castellaniella defragrans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-perillyl alcohol + NAD+ most efficient substrate Castellaniella defragrans ? + NADH + H+
-
?
(S)-perillyl alcohol + NAD+
-
Castellaniella defragrans perillyl aldehyde + NADH
-
?
benzyl alcohol + NAD+
-
Castellaniella defragrans benzaldehyde + NADH + H+
-
?
benzyl alcohol + NAD+
-
Castellaniella defragrans 65Phen benzaldehyde + NADH + H+
-
?
benzyl alcohol + NAD+
-
Castellaniella defragrans ?
-
?
citronellol + NAD+
-
Castellaniella defragrans ?
-
?
citronellol + NAD+
-
Castellaniella defragrans citronellal + NADH + H+
-
?
cumic alcohol + NAD+
-
Castellaniella defragrans ?
-
?
geraniol + dichlorophenolindophenol
-
Castellaniella defragrans ?
-
?
geraniol + NAD+
-
Castellaniella defragrans geranial + NADH + H+
-
?
geraniol + NAD+
-
Castellaniella defragrans 65Phen geranial + NADH + H+
-
?
geraniol + oxidized dichlorophenolindophenol
-
Castellaniella defragrans geranial + reduced dichlorophenolindophenol
-
?
additional information the enzyme cannot use phenazine methosulfate as alternative acceptor Castellaniella defragrans ?
-
?
additional information phenazine methosulfate is not accepted as an electron acceptor Castellaniella defragrans ?
-
?
additional information the enzyme cannot use phenazine methosulfate as alternative acceptor Castellaniella defragrans 65Phen ?
-
?
additional information phenazine methosulfate is not accepted as an electron acceptor Castellaniella defragrans 65Phen ?
-
?
nerol + NAD+
-
Castellaniella defragrans neral + NADH + H+
-
?
nerol + NAD+
-
Castellaniella defragrans 65Phen neral + NADH + H+
-
?
nerol + NAD+
-
Castellaniella defragrans ?
-
?
p-cumic alcohol + NAD+
-
Castellaniella defragrans ? + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38272, calculated Castellaniella defragrans
homodimer 2 * 39000, SDS-PAGE Castellaniella defragrans
homodimer 2 * 38272, calculated from amino acid sequence Castellaniella defragrans

Synonyms

Synonyms Comment Organism
GeDH
-
Castellaniella defragrans
geoA
-
Castellaniella defragrans
geraniol DH
-
Castellaniella defragrans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
Castellaniella defragrans

Cofactor

Cofactor Comment Organism Structure
additional information NADPH is ineffective as a cosubstrate Castellaniella defragrans
NAD+ dependent on, no activity with NADP+ Castellaniella defragrans
NADH enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided Castellaniella defragrans

Expression

Organism Comment Expression
Castellaniella defragrans enzyme activity is induced during growth with limonene in comparison to growth with acetate up
Castellaniella defragrans activitiy is induced during growth with limonene in comparison to growth with acetate up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
citronellol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
100
-
citronellol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
100
-
citronellol pH 9.4, 21°C Castellaniella defragrans
110
-
benzyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
220
-
benzyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
220
-
benzyl alcohol pH 9.4, 21°C Castellaniella defragrans
310
-
nerol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
310
-
nerol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
310
-
nerol pH 9.4, 21°C Castellaniella defragrans
520
-
cumic alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
520
-
p-cumic alcohol pH 9.4, 21°C Castellaniella defragrans
620
-
geraniol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
940
-
cumic alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1570
-
geraniol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
1570
-
geraniol pH 9.4, 21°C Castellaniella defragrans
2020
-
(S)-perillyl alcohol native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans
2020
-
(S)-perillyl alcohol pH 9.4, 21°C Castellaniella defragrans
3550
-
(S)-perillyl alcohol recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C Castellaniella defragrans