KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADPH | isoform 2,5-diketo-D-gluconate reductase I, at pH 7.0 and 30°C | Corynebacterium sp. | |
0.013 | - |
NADPH | isoform 2,5-diketo-D-gluconate reductase II, at pH 7.0 and 30°C | Corynebacterium sp. | |
1.8 | - |
2,5-didehydro-D-gluconate | isoform 2,5-diketo-D-gluconate reductase I, at pH 7.0 and 30°C | Corynebacterium sp. | |
13.5 | - |
2,5-didehydro-D-gluconate | isoform 2,5-diketo-D-gluconate reductase II, at pH 7.0 and 30°C | Corynebacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, isoform 2,5-diketo-D-gluconate reductase I, SDS-PAGE | Corynebacterium sp. |
34000 | - |
1 * 34000, isoform 2,5-diketo-D-gluconate reductase II, SDS-PAGE | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Corynebacterium sp. SHS 0007 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Amicon Matrix Gel Red A gel filtration | Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH + H+ | 100% activity | Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
? | |
2,5-didehydro-D-gluconate + NADPH + H+ | 100% activity | Corynebacterium sp. SHS 0007 | 2-dehydro-L-gulonate + NADP+ | - |
? | |
5-dehydro-D-fructose + NADPH + H+ | isoforms 2,5-diketo-D-gluconate reductase I and II show 150% and 13% activity, respectively, compared to 2,5-didehydro-D-gluconate | Corynebacterium sp. | L-sorbose + NADP+ | - |
? | |
additional information | no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose | Corynebacterium sp. | ? | - |
? | |
additional information | no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose | Corynebacterium sp. SHS 0007 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, isoform 2,5-diketo-D-gluconate reductase I, SDS-PAGE | Corynebacterium sp. |
monomer | 1 * 34000, isoform 2,5-diketo-D-gluconate reductase II, SDS-PAGE | Corynebacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconate reductase I | isoform | Corynebacterium sp. |
2,5-diketo-D-gluconate reductase II | isoform | Corynebacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoform 2,5-diketo-D-gluconate reductase I | Corynebacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
38 | temperature stability of isoform 2,5-diketo-D-gluconate reductase I is measured by storing the enzyme in 0.1 M Tris-HC1 buffer (pH 7.0) for 1 h. The activity of the enzyme decreases gradually with temperature from 0°C to 27°C, falls rapidly above 27°C, and is lost completely at about 38°C. The remaining activity at 27°C is about 77% of that at 0°C | Corynebacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | isoform 2,5-diketo-D-gluconate reductase I | Corynebacterium sp. |
6 | 7.5 | isoform 2,5-diketo-D-gluconate reductase II | Corynebacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | when isoform I is incubated at different pH values for 3 h at 30°C, the remaining activity is about 29% of the original level at pH 5.0, about 65% at pH 6.0 and about 51% at pH 7.0 | Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Corynebacterium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corynebacterium sp. | isoform 2,5-diketo-D-gluconate reductase II, isoelectric focusing | - |
4.1 |
Corynebacterium sp. | isoform 2,5-diketo-D-gluconate reductase I, isoelectric focusing | - |
4.3 |