Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be utilized for preparation of enantiomerically pure (R)-2-hydroxy-4-methylpentanoate in 88% yield, using formate dehydrogenase recycling of the NADH coenzyme | Lacticaseibacillus casei |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
0.12 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
0.13 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
0.2 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
0.4 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
0.6 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
1.6 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
2.3 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
2.7 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
3.3 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxy-4-methylpentanoate + NAD+ | i.e. (R)-2-hydroxyisocaproate | Lacticaseibacillus casei | 4-methyl-2-oxopentanoate + NADH + H+ | i.e. 2-oxoisocaproate | r | |
2-hydroxyhexanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxohexanoate + NADH + H+ | - |
r | |
2-hydroxyoctanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxooctanoate + NADH + H+ | - |
r | |
2-hydroxypentanoate + NAD+ | - |
Lacticaseibacillus casei | 2-oxopentanoate + NADH + H+ | - |
r | |
2-oxohexanoate + NADH + H+ | i.e. 2-oxovalerate | Lacticaseibacillus casei | 2-hydroxyhexanoate + NAD+ | - |
r | |
2-oxooctanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxyoctanoate + NAD+ | - |
r | |
2-oxopentanoate + NADH + H+ | i.e. 2-oxocaproate | Lacticaseibacillus casei | 2-hydroxypentanoate + NAD+ | - |
r | |
3-(4-hydroxyphenyl)-2-oxopropanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxy-3-(4-hydroxyphenyl)propanoate + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NADH + H+ | i.e. 2-oxoisocaproate | Lacticaseibacillus casei | (R)-2-hydroxy-4-methylpentanoate + NAD+ | i.e. (R)-2-hydroxyisocaproate | r | |
phenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | phenyllactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
R-HicDH | - |
Lacticaseibacillus casei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
24 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
26 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
43 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
84 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
139 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
197 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
788 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
968 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
1200 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lacticaseibacillus casei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Lacticaseibacillus casei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
2-hydroxypentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
9.7 | - |
2-hydroxyhexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
18 | - |
(R)-2-hydroxy-4-methylpentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
25 | - |
2-Hydroxyoctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
139 | - |
2-oxooctanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
210 | - |
4-methyl-2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
1100 | - |
2-Oxohexanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
1300 | - |
3-(4-hydroxyphenyl)-2-oxopropanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
1600 | - |
2-oxopentanoate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei | |
4800 | - |
phenylpyruvate | pH 7.0, temperature not specified in the publication | Lacticaseibacillus casei |