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Literature summary for 1.1.1.345 extracted from

  • Hummel, W.; Schütte, H.; Kula, M.-R.
    D-2-Hydroxyisocaproate dehydrogenase from Lactobacillus casei (1985), Appl. Microbiol. Biotechnol., 21, 7-15.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, decrease of 13% in the enzymatic activity Lacticaseibacillus paracasei
Hg2+ 0.1 mM, complete inhibition Lacticaseibacillus paracasei
additional information the addition of citrate and EDTA (up to 10 mM) has no effect on the enzymatic activity. The addition of of iodoacetamide, KCN, 2-mercaptoethanol, dithiothreitol, and reduced glutathione in concentrations up to 20 mM has no effect Lacticaseibacillus paracasei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH pH 7.0, 25°C Lacticaseibacillus paracasei
0.06
-
4-methyl-2-oxopentanoate pH 7.0, 25°C Lacticaseibacillus paracasei
0.11
-
2-oxopentanoate pH 7.0, 25°C Lacticaseibacillus paracasei
0.11
-
2-Oxohexanoate pH 7.0, 25°C Lacticaseibacillus paracasei
0.15
-
phenylpyruvate pH 7.0, 25°C Lacticaseibacillus paracasei
0.5
-
NAD+ pH 7.0, 25°C Lacticaseibacillus paracasei

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no influence on the enzymatic activity can be detected with Mg2+ and Ca2+ and only very weak effects with Cd2+, Co2+ , and Mn2+ Lacticaseibacillus paracasei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Lacticaseibacillus paracasei
74000
-
gel filtration Lacticaseibacillus paracasei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus paracasei P17584
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lacticaseibacillus paracasei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxohexanoate + NADH + H+ i.e. 2-oxocaproate Lacticaseibacillus paracasei 2-hydroxyhexanoate + NAD+
-
r
2-oxopentanoate + NADH + H+ i.e. 2-oxovalerate Lacticaseibacillus paracasei 2-hydroxypentanoate + NAD+
-
r
4-methyl-2-oxopentanoate + NADH + H+ i.e. 2-oxoisocaproate Lacticaseibacillus paracasei (R)-2-hydroxy-4-methylpentanoate + NAD+ i.e. (R)-2-hydroxyisocaproate r
phenylpyruvate + NADH + H+
-
Lacticaseibacillus paracasei phenyllactate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Lacticaseibacillus paracasei

Synonyms

Synonyms Comment Organism
D-2-hydroxyisocaproate dehydrogenase
-
Lacticaseibacillus paracasei
HdhD
-
Lacticaseibacillus paracasei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Lacticaseibacillus paracasei
50
-
-
Lacticaseibacillus paracasei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
60 min, 95% of the activity can be recovered Lacticaseibacillus paracasei
50
-
at temperatures above 50°C the stability of the enzyme decreases drastically Lacticaseibacillus paracasei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 reduction of 4-methyl-2-oxopentanoate Lacticaseibacillus paracasei
7
-
assay at Lacticaseibacillus paracasei
8 9 oxidation of (R)-2-hydroxy-4-methylpentanoate Lacticaseibacillus paracasei

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.3 9.7 6 weeks, more than 80% of the activity is recovered Lacticaseibacillus paracasei

Cofactor

Cofactor Comment Organism Structure
NAD+ with NADP+ no reaction was observed in concentrations up to 4.5 mM Lacticaseibacillus paracasei
NADH rhe enzyme requires NADH as a coenzyme for the hydrogenation reaction, NADPH does not support the reaction in concentrations up to 0.1 mM Lacticaseibacillus paracasei