Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, decrease of 13% in the enzymatic activity | Lacticaseibacillus paracasei | |
Hg2+ | 0.1 mM, complete inhibition | Lacticaseibacillus paracasei | |
additional information | the addition of citrate and EDTA (up to 10 mM) has no effect on the enzymatic activity. The addition of of iodoacetamide, KCN, 2-mercaptoethanol, dithiothreitol, and reduced glutathione in concentrations up to 20 mM has no effect | Lacticaseibacillus paracasei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | pH 7.0, 25°C | Lacticaseibacillus paracasei | |
0.06 | - |
4-methyl-2-oxopentanoate | pH 7.0, 25°C | Lacticaseibacillus paracasei | |
0.11 | - |
2-oxopentanoate | pH 7.0, 25°C | Lacticaseibacillus paracasei | |
0.11 | - |
2-Oxohexanoate | pH 7.0, 25°C | Lacticaseibacillus paracasei | |
0.15 | - |
phenylpyruvate | pH 7.0, 25°C | Lacticaseibacillus paracasei | |
0.5 | - |
NAD+ | pH 7.0, 25°C | Lacticaseibacillus paracasei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no influence on the enzymatic activity can be detected with Mg2+ and Ca2+ and only very weak effects with Cd2+, Co2+ , and Mn2+ | Lacticaseibacillus paracasei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Lacticaseibacillus paracasei |
74000 | - |
gel filtration | Lacticaseibacillus paracasei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus paracasei | P17584 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Lacticaseibacillus paracasei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxohexanoate + NADH + H+ | i.e. 2-oxocaproate | Lacticaseibacillus paracasei | 2-hydroxyhexanoate + NAD+ | - |
r | |
2-oxopentanoate + NADH + H+ | i.e. 2-oxovalerate | Lacticaseibacillus paracasei | 2-hydroxypentanoate + NAD+ | - |
r | |
4-methyl-2-oxopentanoate + NADH + H+ | i.e. 2-oxoisocaproate | Lacticaseibacillus paracasei | (R)-2-hydroxy-4-methylpentanoate + NAD+ | i.e. (R)-2-hydroxyisocaproate | r | |
phenylpyruvate + NADH + H+ | - |
Lacticaseibacillus paracasei | phenyllactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Lacticaseibacillus paracasei |
Synonyms | Comment | Organism |
---|---|---|
D-2-hydroxyisocaproate dehydrogenase | - |
Lacticaseibacillus paracasei |
HdhD | - |
Lacticaseibacillus paracasei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lacticaseibacillus paracasei |
50 | - |
- |
Lacticaseibacillus paracasei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
60 min, 95% of the activity can be recovered | Lacticaseibacillus paracasei |
50 | - |
at temperatures above 50°C the stability of the enzyme decreases drastically | Lacticaseibacillus paracasei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | reduction of 4-methyl-2-oxopentanoate | Lacticaseibacillus paracasei |
7 | - |
assay at | Lacticaseibacillus paracasei |
8 | 9 | oxidation of (R)-2-hydroxy-4-methylpentanoate | Lacticaseibacillus paracasei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.3 | 9.7 | 6 weeks, more than 80% of the activity is recovered | Lacticaseibacillus paracasei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | with NADP+ no reaction was observed in concentrations up to 4.5 mM | Lacticaseibacillus paracasei | |
NADH | rhe enzyme requires NADH as a coenzyme for the hydrogenation reaction, NADPH does not support the reaction in concentrations up to 0.1 mM | Lacticaseibacillus paracasei |