Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.345 extracted from

  • Tamura, Y.; Ohkubo, A.; Iwai, S.; Wada, Y.; Shinoda, T.; Arai, K.; Mineki, S.; Iida, M.; Taguchi, H.
    Two forms of NAD-dependent D-mandelate dehydrogenase in Enterococcus faecalis IAM 10071 (2002), Appl. Environ. Microbiol., 68, 947-951.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
4-methyl-2-oxopentanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
0.15
-
3-methyl-2-oxobutanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
0.26
-
phenylglyoxylate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
0.4
-
2-oxopentanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
0.6
-
2-Oxohexanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
0.76
-
(R)-mandelate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
3.4
-
2-oxo-3-phenylpropanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
4
-
2-oxobutanoate pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis
40000
-
x * 40000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
110000
-
gel filtration, D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis
130000
-
gel filtration, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
3.7
-
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelate + NAD+ Vmax/Km is 0.4% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis phenylglyoxylate + NADH + H+
-
?
(R)-mandelate + NAD+ Vmax/Km is 1% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis phenylglyoxylate + NADH + H+
-
?
2-oxo-3-phenylpropanoate + NADH + H+ Vmax/KM is 1.9% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxy-3-phenylpropanoate + NAD+
-
?
2-oxo-3-phenylpropanoate + NADH + H+ Vmax/Km is 2.7% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxy-3-phenylpropanoate + NAD+
-
?
2-oxobutanoate + NADH + H+ Vmax/Km is 0.25% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxybutanoate + NAD+
-
?
2-oxobutanoate + NADH + H+ Vmax/KM is less than 1% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxybutanoate + NAD+
-
?
2-oxohexanoate + NADH + H+ Vmax/Km is 4.1% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxyhexanoate + NAD+
-
?
2-oxohexanoate + NADH + H+ Vmax/Km is 5.1% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxyhexanoate + NAD+
-
?
2-oxopentanoate + NADH + H+ Vmax/Km is 5.8% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxypentanoate + NAD+
-
?
2-oxopentanoate + NADH + H+ Vmax/Km is 7.6% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 2-hydroxypentanoate + NAD+
-
?
3-methyl-2-oxobutanoate + NADH + H+ Vmax/Km is 51% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 3-methyl-2-hydroxybutanoate + NAD+
-
?
3-methyl-2-oxobutanoate + NADH + H+ Vmax/Km is 85.7% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis 3-methyl-2-hydroxybutanoate + NAD+
-
?
4-methyl-2-oxopentanoate + NADH + H+ highest Vmax/Km value of all substrates tested Enterococcus faecalis 4-methyl-2-hydroxypentanoate + NAD+
-
?
additional information both enzymes (D-mandelate dehydrogenase D-ManDH1 and D-mandelate dehydrogenase D-ManDH2) exhibit no or very little activity toward small 2-ketoacid substrates, such as pyruvate, hydroxypyruvate, and 2-ketobutyrate, and much higher activity toward substrates with larger aliphatic or aromatic side chains. The two enzymes exhibit higher activity (smaller Km and larger Vmax) for 2-ketoacid substrates branched at the C3 or C4 position than for unbranched substrates; i.e., 2-ketoisovalerate and 2-ketoisocaproate are more favorable than 2-ketovalerate and 2-ketocaproate, respectively. Among aromatic substrates, the two enzymes preferr benzoylformate to phenylpyruvate by 9- and 17-fold, respectively Enterococcus faecalis ?
-
?
additional information both enzymes (D-mandelate dehydrogenase D-ManDH1 and D-mandelate dehydrogenase D-ManDH2) exhibit no or very little activity toward small 2-oxoacid substrates, such as pyruvate, hydroxypyruvate, and 2-oxobutyrate, and much higher activity toward substrates with larger aliphatic or aromatic side chains. The two enzymes exhibit higher activity (smaller Km and larger Vmax) for 2-ketoacid substrates branched at the C3 or C4 position than for unbranched substrates; i.e., 2-oxoisovalerate and 2-oxoisocaproate are more favorable than 2-oxovalerate and 2-oxocaproate, respectively. Among aromatic substrates, the two enzymes prefer benzoylformate to phenylpyruvate by 9- and 17-fold, respectively Enterococcus faecalis ?
-
?
phenylglyoxylate + NADH + H+ Vmax/Km is 16.7% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis hydroxy(phenyl)acetic acid + NAD+
-
?
phenylglyoxylate + NADH + H+ Vmax/Km is 44% compared to 4-methyl-2-oxopentanoate Enterococcus faecalis hydroxy(phenyl)acetic acid + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis
? x * 40000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis

Synonyms

Synonyms Comment Organism
D-mandelate dehydrogenase
-
Enterococcus faecalis
D-mandelate dehydrogenase misleading Enterococcus faecalis
D-ManDH1 the strain contains at least two distinct forms of D-ManDH, the two apparent D-mandelate dehydrogenases are named D-ManDH1 and D-ManDH2 according to the order of their elution Enterococcus faecalis
D-ManDH2 the strain contains at least two distinct forms of D-ManDH, the two apparent D-mandelate dehydrogenases are named D-ManDH1 and D-ManDH2 according to the order of their elution Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Enterococcus faecalis
30 40 D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis
35 45 D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
maximal catalytic efficiency, D-mandelate dehydrogenase D-ManDH1 Enterococcus faecalis
4.5
-
maximal catalytic efficiency, D-mandelate dehydrogenase D-ManDH2 Enterococcus faecalis
7.5
-
assay at Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
NADH NADH can not be replaced by NADPH in the benzoylformate reduction by D-mandelate dehydrogenase D-ManDH1 or D-ManDH2 Enterococcus faecalis