Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.343 extracted from

  • Huang, R.; Chen, H.; Zhong, C.; Kim, J.E.; Zhang, Y.H.
    High-throughput screening of coenzyme preference change of thermophilic 6-phosphogluconate dehydrogenase from NADP+ to NAD+ (2016), Sci. Rep., 6, 32644.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.343

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOP10 and BL21(DE3) cells Moorella thermoacetica

Protein Variants

Protein Variants Comment Organism
A30C/R31I/T32K the catalytic efficiency of the mutant for NAD+ is increased by about 1.6fold compared to the wild type enzyme Moorella thermoacetica
A30D/R31I/T32I the catalytic efficiency of the mutant for NAD+ is increased by 54fold, with a 4278fold reversal of coenzyme selectivity from NADP+ to NAD+ Moorella thermoacetica
A30E/R31I/T32D the catalytic efficiency of the mutant for NAD+ is increased by about 9.5fold compared to the wild type enzyme Moorella thermoacetica
R31I the catalytic efficiency of the mutant for NAD+ is increased by about 4.7fold compared to the wild type enzyme Moorella thermoacetica
R31I/T32G the catalytic efficiency of the mutant for NAD+ is increased by about 4fold compared to the wild type enzyme Moorella thermoacetica
R31T the catalytic efficiency of the mutant for NAD+ is increased by about 1.7fold compared to the wild type enzyme Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01187
-
 NAD+ mutant enzyme A30D/R31I/T32I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
0.0404
-
 NAD+ mutant enzyme A30C/R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
0.127
-
 NAD+ mutant enzyme A30E/R31I/T32D, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
0.354
-
 NAD+ mutant enzyme R31I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
0.362
-
 NAD+ mutant enzyme R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
0.605
-
 NAD+ mutant enzyme R31T, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
1.397
-
 NAD+ wild type enzyme, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD+ Moorella thermoacetica the wild type enzyme shows very low activity with NAD+, mutations at A30, R31, and T32 increase the catalytic activity with NAD+ D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica A0A1J5NX54
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+ the wild type enzyme shows very low activity with NAD+, mutations at A30, R31, and T32 increase the catalytic activity with NAD+ Moorella thermoacetica D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Moorella thermoacetica
6PGDH
-
 Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.75
-
 NAD+ mutant enzyme A30D/R31I/T32I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
6
-
 NAD+ mutant enzyme A30C/R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
9.12
-
 NAD+ mutant enzyme R31T, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
10.8
-
 NAD+ mutant enzyme A30E/R31I/T32D, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
12.6
-
 NAD+ wild type enzyme, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
12.94
-
 NAD+ mutant enzyme R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
15.01
-
 NAD+ mutant enzyme R31I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Moorella thermoacetica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 NAD+ wild type enzyme, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
14.8
-
 NAD+ mutant enzyme A30C/R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
15
-
 NAD+ mutant enzyme R31T, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
35.8
-
 NAD+ mutant enzyme R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
42.4
-
 NAD+ mutant enzyme R31I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
85.1
-
 NAD+ mutant enzyme A30E/R31I/T32D, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica
484.2
-
 NAD+ mutant enzyme A30D/R31I/T32I, in 100 mM HEPES (pH 7.5), at 50°C Moorella thermoacetica