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Literature summary for 1.1.1.34 extracted from

  • Tabernero, L.; Rodwell, V.W.; Stauffacher, C.V.
    Crystal structure of a statin bound to a class II hydroxymethylglutaryl-CoA reductase (2003), J. Biol. Chem., 278, 19933-19938.
    View publication on PubMed
Search for more literature for 1.1.1.34

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with inhibitor lovastatin, from 1.2 M ammonium sulfate, at 20°C, a few days, larger crystals by microseeding, complexing with the inhibitor by soaking in a KOH solution, pH 9.0, containing 2 mM lovastatin, X-ray diffraction structure determination and analysis at 2.6 A resolution Pseudomonas mevalonii

Inhibitors

Inhibitors Comment Organism Structure
lovastatin slightly inhibitory statin for class II enzyme, binding structure at the active site involves the residues Lys267, Asn271, Glu83, Arg261, and 2 water molecules, substrate mimicking binding mode Pseudomonas mevalonii

Organism

Organism UniProt Comment Textmining
Pseudomonas mevalonii
-
 class II enzyme
-

Reaction

Reaction Comment Organism Reaction ID
(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ via mevaldehyde as an intermediate product, enzyme contains a catalytic His381, Lys267 is also involved in catalysis, active site structure Pseudomonas mevalonii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ overall reaction Pseudomonas mevalonii (R)-mevalonate + CoA + 2 NADP+
-
 r
(S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ first step reaction Pseudomonas mevalonii mevaldyl-CoA + NADP+
-
 r
mevaldehyde + NADPH + H+ third step reaction Pseudomonas mevalonii (R)-mevalonate + NADP+
-
 r
mevaldyl-CoA + H2O second step reaction Pseudomonas mevalonii mevaldehyde + NADP+
-
 r

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl CoA reductase
-
 Pseudomonas mevalonii