Cloned (Comment) | Organism |
---|---|
isozyme HMGR1, DNA and amino acid sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system as His-tagged enzyme | Homarus americanus |
isozyme HMGR2, DNA and amino acid sequence determination and analysis | Homarus americanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lovastatin | - |
Homarus americanus | |
mevalonic acid | - |
Homarus americanus | |
additional information | no inhibition by farnesoate and farnesoic acid | Homarus americanus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound, isozyme HMGR2, about 25% of total enzyme content | Homarus americanus | 16020 | - |
soluble | isozyme HMGR1, about 75% of total enzyme content | Homarus americanus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
- |
Homarus americanus |
140000 | - |
isozyme HMGR1, minor peak, gel filtration | Homarus americanus |
540000 | - |
isozyme HMGR1, major peak, gel filtration | Homarus americanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homarus americanus | Q7YT62 | lobster, 2 enzyme forms: a soluble and a membrane-bound enzyme | - |
Homarus americanus | Q7YT63 | lobster, 2 enzyme forms: a soluble isozyme HMGR1 and a membrane-bound isozyme HMGR2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the isozyme is not regulated by phosphorylation | Homarus americanus |
additional information | the isozyme is probably not regulated by phosphorylation | Homarus americanus |
Purification (Comment) | Organism |
---|---|
native soluble isozyme HMGR1 from mandibular organ by ultracentrifugation, anion exchange chromatography, ultrafiltration and gel filtration, recombinant enzyme from Sf9 insect cells by nickel affinity chromatography | Homarus americanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mandibular organ | - |
Homarus americanus | - |
additional information | very low content in other tissues, higher enzyme activity in male organisms than in female, tissue distribution overview | Homarus americanus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0006 | - |
about, native cells | Homarus americanus |
60 | - |
recombinant Sf9 cells | Homarus americanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
Homarus americanus | (R)-mevalonate + CoA + 2 NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
oligomer | 1 x 69000, recombinant His-tagged isozyme HMGR1, SDS-PAGE | Homarus americanus |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl CoA reductase | - |
Homarus americanus |
HMGR | - |
Homarus americanus |
HMGR1 | - |
Homarus americanus |
HMGR2 | - |
Homarus americanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homarus americanus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000045 | - |
lovastatin | pH 7.4, recombinant isozyme HMGR1 | Homarus americanus | |
0.081 | - |
mevalonic acid | pH 7.4, recombinant isozyme HMGR1 | Homarus americanus |