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Literature summary for 1.1.1.34 extracted from

  • Kim, D.Y.; Bochar, D.A.; Stauffacher, C.V.; Rodwell, V.W.
    Expression and characterization of the HMG-CoA reductase of the thermophilic archaeon Sulfolobus solfataricus (1999), Protein Expr. Purif., 17, 435-442.
    View publication on PubMed
Search for more literature for 1.1.1.34

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of the Sulfolobus solfataricus hmgA gene in Escherichia coli with the argU gene that encodes tRNAAGA,AGG results in an over 10fold increase in enzyme yield Saccharolobus solfataricus
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
synthesis due to use of rare codon, expression of enzyme in Escherichia coli is poor. Coexpression of the S. solfataricus hmgA gene with the argU gene that encodes tRNAAGA,AGG resulted in an over 10-fold increase in enzyme yield Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
Mevinolin competitive with 3-hydroxy-3-methylglutaryl-CoA Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus
-
 (R)-mevalonate + CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
 pH 5.5, 50°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Saccharolobus solfataricus
9.5
-
 oxidative acylation of (R,S)-mevaldehyde or mevalonate Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
 Mevinolin pH 5.5, 50°C Saccharolobus solfataricus