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Literature summary for 1.1.1.337 extracted from

  • Feil, I.K.; Lerch, H.P.; Schomburg, D.
    Deletion variants of L-hydroxyisocaproate dehydrogenase. Probing substrate specificity (1994), Eur. J. Biochem., 223, 857-863.
    View publication on PubMed
Search for more literature for 1.1.1.337

Protein Variants

Protein Variants Comment Organism
del I81/delK82 phenylpyruvate is the only substrate which is converted at a significant catalytic rate by the mutant enzyme. In the publication this mutant is referred to as Ile100ADELTA/Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
del I81/delK82/delN87/delP88 specific modifications in catalytic rates and substrate recognition Weissella confusa
del L 83 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Leu101DELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
delI81 drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Ile100ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
delK82 drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
delN87 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Asn105ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
delN87/delP88 for the deletion mutant enzyme 2-oxo carboxylic acids branched at C4 are better substrates than 2-oxocaproate, the substrate with the best kcat,/KM ratio known for the wild-type enzyme.The mutation results in a 5.2fold increased catalytic efficiency towards 4-methyl-2-oxopentanoate compared to the wild-type enzyme. In the publication this mutant is referred to as Asn105ADELTA/Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
delP88 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
additional information the coenzyme loop, a functional element which is essential for catalysis and substrate specificity, is modified in order to identify the residues involved in the catalytic reaction and substrate specificity Weissella confusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
0.067
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
0.071
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
0.1 1 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa
0.1 2 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
0.17
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
0.33
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
0.41
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
0.62
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
0.9 1 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1 4 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
2
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
2 5 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
2.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
2.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
2.8
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
3.3
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
3.5
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
5
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
5.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
6.4
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
9.3
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
11
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
18
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
18
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
19
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
19
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
27
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
30
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
43
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
50
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
62
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
110
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
140
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
200
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa

Organism

Organism UniProt Comment Textmining
Weissella confusa P14295 i.e. Lactobacillus confusus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Weissella confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxocaproate + NADH + H+
-
 Weissella confusa 2-hydroxycaproate + NAD+
-
 r
4-methyl-2-oxopentanoate + NADH + H+
-
 Weissella confusa (S)-2-hydroxy-4-methylpentanoate + NAD+
-
 r
phenylpyruvate + NADH + H+
-
 Weissella confusa 3-phenyllactate + NAD+
-
 r
pyruvate + NADH + H+
-
 Weissella confusa L-lactate + NAD+
-
 r

Synonyms

Synonyms Comment Organism
L-2-hydroxyisocaproate dehydrogenase
-
 Weissella confusa
L-HicDH
-
 Weissella confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Weissella confusa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
0.03
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
0.23
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
0.63
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
0.76
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.7
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
13
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
15
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
17
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
20
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
25
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
39
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
40
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
46
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
90
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
280
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
490
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
620
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
750
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
920
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1200
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
2400
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
2600
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
4200
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
4400
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
7300
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
12000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
15000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
19000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
21000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
89000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
360000
-
 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa
520000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Weissella confusa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00013
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
0.00021
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
0.0038
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
0.025
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
0.035
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
0.092
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
0.32
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
0.6
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
0.63
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
0.68
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
0.91
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
0.92
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.4
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.8
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
8
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
8.4
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
15
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
26
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
160
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
380
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
790
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
2200
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
2900
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
4300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
4300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
6300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
8700
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
16000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
34000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
36000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
46000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
190000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
270000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
280000
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
3300000
-
 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa