Application | Comment | Organism |
---|---|---|
synthesis | synthesis of preparative quantities of 2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid and 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid by coupled reaction of enzyme WbpB and transaminase WpbE | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | G3XD23 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 3 weeks | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetamido-2-deoxy-alpha-D-glucuronic acid + NAD+ + L-glutamate | activity of enzyme His6-WbpB is detected only in presence of transaminase WbpE, and vice versa | Pseudomonas aeruginosa | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid + NADH + 2-ketoglutarate | product of the coupled reaction of enzyme WbpB and transaminase WbpE | ? |