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Literature summary for 1.1.1.334 extracted from
- Plant tropane alkaloid biosynthesis evolved independently in the Solanaceae and Erythroxylaceae (2012), Proc. Natl. Acad. Sci. USA, 109, 10304-10309.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Erythroxylum coca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, inhibition by less than 30% | Erythroxylum coca |  |
| Cu2+ | 5 mM, 8% residual activity | Erythroxylum coca | |
| Fe2+ | 5 mM, inhibition by less than 30% | Erythroxylum coca | |
| methyl ecgonine | - |
Erythroxylum coca | |
| methyl ecgonone | - |
Erythroxylum coca | |
| NADP+ | - |
Erythroxylum coca | |
| NADPH | - |
Erythroxylum coca | |
| Zn2+ | 5 mM, 58% residual activity | Erythroxylum coca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
NADPH | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.0009 | - |
NADP+ | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.069 | - |
methyl ecgonone | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.151 | - |
methyl ecgonine | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by monovalent or divalent cations | Erythroxylum coca |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36910 | - |
calculated from the amino acid sequence | Erythroxylum coca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl ecgonone + NADPH + H+ | Erythroxylum coca | cocaine biosynthesis | methyl ecgonine + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erythroxylum coca | E7C196 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Erythroxylum coca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | less than 20% of activity compared to young leaves | Erythroxylum coca | - |
| leaf | highest activity in young leaves | Erythroxylum coca | - |
| mesophyll | of young leaves | Erythroxylum coca | - |
| additional information | no activity in mature leaves or in roots | Erythroxylum coca | - |
| seed | - |
Erythroxylum coca | - |
| stem | less than 20% of activity compared to young leaves | Erythroxylum coca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0002669 | - |
pH 6.8 temperature not specified in the publication | Erythroxylum coca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxytropinone + NADPH + H+ | reduction at 45% of the activity with methylecgonone | Erythroxylum coca | ? + NADP+ | - |
r | |
| methyl ecgonone + NADH + H+ | NADH can substitute for NADPH with 14% of the activity compared to NADPH | Erythroxylum coca | methyl ecgonine + NAD+ | - |
r | |
| methyl ecgonone + NADPH + H+ | - |
Erythroxylum coca | methyl ecgonine + NADP+ | - |
r | |
| methyl ecgonone + NADPH + H+ | cocaine biosynthesis | Erythroxylum coca | methyl ecgonine + NADP+ | - |
r | |
| additional information | no reduction of 8-thiabicyclo[3.2.1]octan-3-one, cyclohexanone, cyclooctanone, N-methyl-4-piperidone | Erythroxylum coca | ? | - |
? | |
| nortropinone + NADPH + H+ | reduction at 6% of the activity with methylecgonone | Erythroxylum coca | ? + NADP+ | - |
r | |
| tropinone + NADPH + H+ | reduction at 36% of the activity with methylecgonone | Erythroxylum coca | pseudotropine + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MecgoR | - |
Erythroxylum coca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
30 min, more than 30% loss of activity | Erythroxylum coca |
| 37 | - |
30 min, more than 70% loss of activity | Erythroxylum coca |
| 50 | - |
30 min, complete inactivation abvoe 50°C | Erythroxylum coca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
reduction of methyl ecgonone | Erythroxylum coca |
| 9.8 | - |
oxidation of methyl ecgonine | Erythroxylum coca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 14% of the activity with NADPH | Erythroxylum coca | |
| NADPH | - |
Erythroxylum coca | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001136 | - |
methyl ecgonone | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.00248 | - |
NADPH | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.002673 | - |
methyl ecgonine | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
| 0.506 | - |
NADP+ | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
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Release 2023.1 (January 2023)
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