Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Erythroxylum coca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, inhibition by less than 30% | Erythroxylum coca | |
Cu2+ | 5 mM, 8% residual activity | Erythroxylum coca | |
Fe2+ | 5 mM, inhibition by less than 30% | Erythroxylum coca | |
methyl ecgonine | - |
Erythroxylum coca | |
methyl ecgonone | - |
Erythroxylum coca | |
NADP+ | - |
Erythroxylum coca | |
NADPH | - |
Erythroxylum coca | |
Zn2+ | 5 mM, 58% residual activity | Erythroxylum coca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
NADPH | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.0009 | - |
NADP+ | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.069 | - |
methyl ecgonone | pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.151 | - |
methyl ecgonine | pH 6.8, temperature not specified in the publication | Erythroxylum coca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by monovalent or divalent cations | Erythroxylum coca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36910 | - |
calculated from the amino acid sequence | Erythroxylum coca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl ecgonone + NADPH + H+ | Erythroxylum coca | cocaine biosynthesis | methyl ecgonine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erythroxylum coca | E7C196 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Erythroxylum coca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | less than 20% of activity compared to young leaves | Erythroxylum coca | - |
leaf | highest activity in young leaves | Erythroxylum coca | - |
mesophyll | of young leaves | Erythroxylum coca | - |
additional information | no activity in mature leaves or in roots | Erythroxylum coca | - |
seed | - |
Erythroxylum coca | - |
stem | less than 20% of activity compared to young leaves | Erythroxylum coca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002669 | - |
pH 6.8 temperature not specified in the publication | Erythroxylum coca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxytropinone + NADPH + H+ | reduction at 45% of the activity with methylecgonone | Erythroxylum coca | ? + NADP+ | - |
r | |
methyl ecgonone + NADH + H+ | NADH can substitute for NADPH with 14% of the activity compared to NADPH | Erythroxylum coca | methyl ecgonine + NAD+ | - |
r | |
methyl ecgonone + NADPH + H+ | - |
Erythroxylum coca | methyl ecgonine + NADP+ | - |
r | |
methyl ecgonone + NADPH + H+ | cocaine biosynthesis | Erythroxylum coca | methyl ecgonine + NADP+ | - |
r | |
additional information | no reduction of 8-thiabicyclo[3.2.1]octan-3-one, cyclohexanone, cyclooctanone, N-methyl-4-piperidone | Erythroxylum coca | ? | - |
? | |
nortropinone + NADPH + H+ | reduction at 6% of the activity with methylecgonone | Erythroxylum coca | ? + NADP+ | - |
r | |
tropinone + NADPH + H+ | reduction at 36% of the activity with methylecgonone | Erythroxylum coca | pseudotropine + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MecgoR | - |
Erythroxylum coca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
30 min, more than 30% loss of activity | Erythroxylum coca |
37 | - |
30 min, more than 70% loss of activity | Erythroxylum coca |
50 | - |
30 min, complete inactivation abvoe 50°C | Erythroxylum coca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
reduction of methyl ecgonone | Erythroxylum coca |
9.8 | - |
oxidation of methyl ecgonine | Erythroxylum coca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 14% of the activity with NADPH | Erythroxylum coca | |
NADPH | - |
Erythroxylum coca |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001136 | - |
methyl ecgonone | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.00248 | - |
NADPH | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.002673 | - |
methyl ecgonine | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca | |
0.506 | - |
NADP+ | substrate inhibition, pH 6.8, temperature not specified in the publication | Erythroxylum coca |