Literature summary for 1.1.1.332 extracted from

Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.
Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3 (2010), Org. Biomol. Chem., 8, 3500-3508.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Aspergillus fumigatus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	485 residual activity	Aspergillus fumigatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.27	-	chanoclavine-I	pH 7.5, 37°C	Aspergillus fumigatus
1.1	-	NAD+	pH 7.5, 37°C	Aspergillus fumigatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	addition to EDTA-treated enzyme restores activity to 73% of initial activity	Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein	Aspergillus fumigatus
118000	-	gel filtration	Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	D3J0Z1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chanoclavine-I + NAD+	incubation of enzyme with chanoclavine-I aldehyde in the presence of NADH does not result in detectable chanoclavine-I, indicating a much lower reaction velocity of the reverse reaction	Aspergillus fumigatus	chanoclavine-I aldehyde + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4* 27800, calculated, 4 * 30000, SDS-PAGE of His-tagged recombinant protein	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
fgaDH	-	Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.38	-	chanoclavine-I	pH 7.5, 37°C	Aspergillus fumigatus

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no cofactors: NADP+, FAD, FMN	Aspergillus fumigatus
NAD+	specific for NAD+	Aspergillus fumigatus

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Release 2023.1 (January 2023)