Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium aminobarbitone | 1 mM, 48% inhibition, noncompetitive | Sus scrofa | |
Thiobarbituric acid | 1 mM, 22% inhibition, noncompetitive | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NADPH | - |
Sus scrofa | |
0.15 | - |
4-cyanobenzaldehyde | - |
Sus scrofa | |
0.26 | - |
(3RS)-mevaldate | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH | Sus scrofa | - |
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, calcium phosphate gel, gel filtration | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.573 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 3 months, 0% loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3RS)-mevaldate + NADPH + H+ | - |
Sus scrofa | (R)-mevalonate + NADP+ | - |
r | |
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH | - |
Sus scrofa | (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+ | - |
ir | |
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH | - |
Sus scrofa | (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+ | - |
ir | |
4-cyanobenzaldehyde + NADPH | - |
Sus scrofa | 4-cyanobenzylalcohol + NADP+ | - |
ir | |
pyridine-3-carboxaldehyde + NADPH | - |
Sus scrofa | pyridine-3-methanol + NADP+ | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | giving one-fifth of the rate obtained with NADPH | Sus scrofa | |
NADPH | - |
Sus scrofa |