Crystallization (Comment) | Organism |
---|---|
powdered ammonium sulfate is added to the enzyme solution until it becomes slightly turbid, after 4 days crystallization is complete | Diutina rugosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | - |
Diutina rugosa | |
0.37 | - |
L-3-hydroxyisobutyrate | - |
Diutina rugosa | |
1.25 | - |
D-3-hydroxyisobutyrate | - |
Diutina rugosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
alpha2, 2 * 40000, SDS-PAGE | Diutina rugosa |
80000 | - |
gel filtration | Diutina rugosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diutina rugosa | - |
IFO 0750 | - |
Purification (Comment) | Organism |
---|---|
- |
Diutina rugosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-hydroxyisobutyrate + NAD+ | - |
Diutina rugosa | (S)-methylmalonate semialdehyde + NADH + H+ | - |
r | |
L-3-hydroxyisobutyrate + NAD+ | - |
Diutina rugosa | (S)-methylmalonate semialdehyde + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, 2 * 40000, SDS-PAGE | Diutina rugosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Diutina rugosa |