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Literature summary for 1.1.1.304 extracted from
- Production of S-acetoin from diacetyl by Escherichia coli transformant cells that express the diacetyl reductase gene of Paenibacillus polymyxa ZJ-9 (2013), Lett. Appl. Microbiol., 57, 274-281.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is used for production of S-acetoin with higher than 99.9% optical purity from diacetyl using whole cells of engineered Escherichia coli | Paenibacillus polymyxa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dar, fucntional expression in Escherichia coli strain Rosetta (DE3), resulting in production of S-acetoin with higher than 99.9% optical purity from diacetyl | Paenibacillus polymyxa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
4 * 28500, recombinant enzyme, SDS-PAGE | Paenibacillus polymyxa |
| 118000 | - |
recombinant enzyme, gel filtration | Paenibacillus polymyxa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Paenibacillus polymyxa | the enzyme also catalyzes the stereospcific reaction of (S)-acetoin reduction to butanediol, EC 1.1.1.76 | ? | - |
? |  |
| additional information | Paenibacillus polymyxa ZJ-9 | the enzyme also catalyzes the stereospcific reaction of (S)-acetoin reduction to butanediol, EC 1.1.1.76 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus polymyxa | KC505218 | gene dar | - |
| Paenibacillus polymyxa ZJ-9 | KC505218 | gene dar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain Rosetta (DE3) | Paenibacillus polymyxa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 72.6 | - |
purified recombinant enzyme, NADPH, pH 6.0, 30°C | Paenibacillus polymyxa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacetyl + NADPH + H+ | - |
Paenibacillus polymyxa | (S)-acetoin + NADP+ | - |
ir | |
| diacetyl + NADPH + H+ | - |
Paenibacillus polymyxa ZJ-9 | (S)-acetoin + NADP+ | - |
ir | |
| additional information | the enzyme also catalyzes the stereospcific reaction of (S)-acetoin reduction to butanediol, EC 1.1.1.76 | Paenibacillus polymyxa | ? | - |
? | |
| additional information | the enzyme also catalyzes the stereospcific reaction of (S)-acetoin reduction to butanediol, EC 1.1.1.76 | Paenibacillus polymyxa ZJ-9 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 28500, recombinant enzyme, SDS-PAGE | Paenibacillus polymyxa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAR | - |
Paenibacillus polymyxa |
| diacetyl reductase | - |
Paenibacillus polymyxa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Paenibacillus polymyxa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Paenibacillus polymyxa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inactive with NADH | Paenibacillus polymyxa | |
| NADPH | dependent on | Paenibacillus polymyxa | |
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Release 2023.1 (January 2023)
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