Literature summary for 1.1.1.304 extracted from
Ui, S.; Okajima, Y.; Mimura, A.; Kanai, H.; kobayashi, T.; Kudo, T.
Sequence analysis of the gene for and characterization of D-acetoin forming meso-2,3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli (1997), J. Ferment. Bioeng., 83, 32-37.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Escherichia coli |
Klebsiella pneumoniae |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
26591 |
- |
4 * 26591, calculated |
Klebsiella pneumoniae |
96000 |
- |
gel filtration |
Klebsiella pneumoniae |
Organism
Organism |
UniProt |
Comment |
Textmining |
Klebsiella pneumoniae |
Q48436 |
bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
2,3-pentanedione + NADH + H+ |
77% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin |
Klebsiella pneumoniae |
3-hydroxy-2-pentanone + NAD+ |
- |
? |
|
diacetyl + NADH + H+ |
87% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin |
Klebsiella pneumoniae |
(S)-acetoin + NAD+ |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
tetramer |
4 * 26591, calculated |
Klebsiella pneumoniae |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
7 |
8 |
- |
Klebsiella pneumoniae |
pI Value
Organism |
Comment |
pI Value Maximum |
pI Value |
Klebsiella pneumoniae |
isoelectric focusing |
7.2 |
5.9 |