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Literature summary for 1.1.1.301 extracted from

  • Povelainen, M.; Eneyaskaya, E.V.; Kulminskaya, A.A.; Ivanen, D.R.; Kalkinnen, N.; Neustroev, K.N.; Miasnikov, A.N.
    Biochemical and genetic characterization of a novel enzyme of pentitol metabolism: D-arabitol-phosphate dehydrogenase (2003), Biochem. J., 371, 191-197.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.301

Cloned(Commentary)

Cloned (Comment) Organism
-
 Enterococcus avium

Inhibitors

Inhibitors Comment Organism Structure
EDTA xylulose 5-phosphate does not protect the enzyme from EDTA inactivation. Addition of Mn2+ at concentrations of up to 2 mM results in complete reactivation of APDH Enterococcus avium
Hg2+ 2 mM, complete inactivation Enterococcus avium
PHMB 2 mM, complete inactivation Enterococcus avium
Zn2+ 2 mM, complete inactivation Enterococcus avium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADH 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate Enterococcus avium
0.23
-
 D-xylulose 5-phosphate 20°C, pH 7.2, cofactor: NADH Enterococcus avium
0.24
-
 NADPH 20°C, pH 7.2, cosubstrate: D-xylulose 5-phosphate Enterococcus avium
0.63
-
 D-arabitol 5-phosphate 20°C, pH 8.5, cofactor: NAD+ Enterococcus avium
0.65
-
 D-xylulose 5-phosphate 20°C, pH 7.2, cofactor NADPH Enterococcus avium
0.71
-
 NAD+ 20°C, pH 8.5: cosubstrate: D-arabitol 5-phosphate Enterococcus avium
0.8
-
 NAD+ 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate Enterococcus avium
2.7
-
 NADP+ 20°C, pH 8.5, cosubstrate: D-arabitol 1-phosphate Enterococcus avium
2.9
-
 D-arabitol 1-phosphate 20°C, pH 8.5, cofactor: NAD+ Enterococcus avium
3.6
-
 D-arabitol 1-phosphate 20°C, pH 8.5, cofactor: NADP+ Enterococcus avium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required. Pure APDH contains 4.05 ions of Mn2+ Enterococcus avium
additional information no requirement for Zn2+ Enterococcus avium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE Enterococcus avium
160000
-
 non-denaturing PAGE Enterococcus avium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Enterococcus avium participates in arabitol catabolism via the arabitol phosphate route ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus avium Q8KQL2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus avium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol 1-phosphate + NAD+
-
 Enterococcus avium D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NADP+
-
 Enterococcus avium D-xylulose 5-phosphate + NADPH + H+
-
 r
D-arabitol 5-phosphate + NAD+
-
 Enterococcus avium D-ribulose 5-phosphate + NADH + H+
-
 ?
D-arabitol 5-phosphate + NADP+
-
 Enterococcus avium D-ribulose 5-phosphate + NADPH + H+
-
 ?
additional information participates in arabitol catabolism via the arabitol phosphate route Enterococcus avium ?
-
 ?
additional information no activity with: xylitol 5-phosphate, D-sorbitol, D-mannitol, xylitol, erythrose 4-phosphate and ribose 5-phosphate. APDH kinetics are consistent with a ternary-complex mechanism Enterococcus avium ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Enterococcus avium

Synonyms

Synonyms Comment Organism
APDH
-
 Enterococcus avium
D-arabitol 1-phosphate dehydrogenase
-
 Enterococcus avium
D-arabitol 5-phosphate dehydrogenase
-
 Enterococcus avium
D-arabitol-phosphate dehydrogenase
-
 Enterococcus avium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.4 reduction of D-xylulose 5-phosphate with NADH or NADPH Enterococcus avium
8.3 8.6 oxidation of D-arabitol 1-phosphate with NAD+ or NADP+ Enterococcus avium

Cofactor

Cofactor Comment Organism Structure
NAD+ the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
NADH the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
NADP+ the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium
NADPH the rates of both reductive and oxidative reactions with NAD+ and NADH as cofactors are about 14 times higher than with NADP+ and NADPH Enterococcus avium

pI Value

Organism Comment pI Value Maximum pI Value
Enterococcus avium isoelectric focusing
-
 6.4