Cloned (Comment) | Organism |
---|---|
gene hom, recombinant expression of His-tagged enzyme in Echerichia coli strain Rosetta (DE3) pLysS | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified enzyme, crystallization at different pH values in the range of pH 6.0-8.5, hanging drop and sitting drop methods of vapour diffusion, 8 mg/ml protein solution is mixed with reservoir solution, different conditions involving addition of 0.2 M magnesium acetate and PEG 3350 or 8000, and 3.5% glycerol, overview. X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Staphylococcus aureus | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | Staphylococcus aureus | - |
L-aspartate 4-semialdehyde + NADPH + H+ | - |
r | |
L-homoserine + NADP+ | Staphylococcus aureus COL | - |
L-aspartate 4-semialdehyde + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A0H2WVX4 | - |
- |
Staphylococcus aureus COL | A0A0H2WVX4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | - |
Staphylococcus aureus | L-aspartate 4-semialdehyde + NADPH + H+ | - |
r | |
L-homoserine + NADP+ | - |
Staphylococcus aureus COL | L-aspartate 4-semialdehyde + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
hom | - |
Staphylococcus aureus |
HSD | - |
Staphylococcus aureus |
SACOL1362 | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Staphylococcus aureus | |
NADPH | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | homoserine dehydrogenase (HSD) is an oxidoreductase that is involved in the reversible conversion of L-aspartate semialdehyde to L-homoserine in a dinucleotide cofactor-dependent reduction reaction. HSD is thus a crucial intermediate enzyme linked to the biosynthesis of several essential amino acids such as lysine, methionine, isoleucine and threonine | Staphylococcus aureus |
physiological function | the enzyme is involved in cell-wall maintenance and essential amino acid biosynthesis. Homoserine dehydrogenase catalyzes a reaction at the branch point of the pathway leading to lysine biosynthesis. This pathway is also referred to as the diaminopimelate (dap) pathway | Staphylococcus aureus |