Literature summary for 1.1.1.3 extracted from

Ejim, L.; Mirza, I.A.; Capone, C.; Nazi, I.; Jenkins, S.; Chee, G.L.; Berghuis, A.M.; Wright, G.D.
New phenolic inhibitors of yeast homoserine dehydrogenase (2004), Bioorg. Med. Chem., 12, 3825-3830.

Application

Application	Comment	Organism
pharmacology	enzyme is a target for inhibitor design for construction of antimicrobial agents	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
10 mg/ml purified recombinant enzyme, in TAPS, pH 8.5, in complex with inhibitor 4,4'-thiobis[2-(1-methylethyl)-phenol] in a molar ratio of 1:1, precipitant solution contains either 0.1 CHES, pH 9.5, 35% PEG 600 or 0.1 M CHES, pH 8.5, 40% PEG 400, and 0.2 M NaCl, 0.005 ml protein complex solution are equilibrated against 0.7 ml of precipitant solution using sitting drop technique, 3 months, X-ray diffraction structure determination and analysis at 3.0 A resolution, modeling	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

10 mg/ml purified recombinant enzyme, in TAPS, pH 8.5, in complex with inhibitor 4,4'-thiobis[2-(1-methylethyl)-phenol] in a molar ratio of 1:1, precipitant solution contains either 0.1 CHES, pH 9.5, 35% PEG 600 or 0.1 M CHES, pH 8.5, 40% PEG 400, and 0.2 M NaCl, 0.005 ml protein complex solution are equilibrated against 0.7 ml of precipitant solution using sitting drop technique, 3 months, X-ray diffraction structure determination and analysis at 3.0 A resolution, modeling

Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism
2,2'-[thiobis[[2-(1,1-dimethylethyl)-5-methyl-4,1-phenylene]oxy]]bis-acetic acid diethyl ester	-	Saccharomyces cerevisiae
4,4'-thiobis[2-(1,1-dimethylethyl)]-5-methyl-phenol	-	Saccharomyces cerevisiae
4,4'-thiobis[2-(1,1-dimethylethyl)]-phenol	-	Saccharomyces cerevisiae
4,4'-thiobis[2-(1-methylethyl)]-phenol	-	Saccharomyces cerevisiae
4,4'-thiobis[5-methyl-2-(1-methylethyl)]-phenol	-	Saccharomyces cerevisiae
4,4'-[1,2-ethanediylbis(thio)]bis[2,6-bis(1-methylpropyl)]-phenol	-	Saccharomyces cerevisiae
4,4'-[1,2-ethanediylbis(thio)]bis[2-(1,1-dimethylethyl)-6-methyl]-phenol	-	Saccharomyces cerevisiae
4-(1-methylheptyl)-1,3-benzenediol	-	Saccharomyces cerevisiae
4-[[2-(2-furanyl)ethyl]thio]-phenol	-	Saccharomyces cerevisiae
4-[[[4-(1,1-dimethylethyl)phenyl]thio]methyl]-2,6-bis(1-methylethyl)-phenol	-	Saccharomyces cerevisiae
bis(4-chlorophenyl)ethyloxiranyl-silane	-	Saccharomyces cerevisiae
additional information	no inhibition by [2-(1,1-dimethylethyl)-4-[[5-(1,1-dimethylethyl)-4-hydroxy-2-methylphenyl]thio]-5-methylphenoxy]-acetic acid and 4-amino-butyric acid 2-tert-butyl-4-(3-tert-butyl-4-hydroxy-phenylsulfanyl)-phenyl ester	Saccharomyces cerevisiae
[2-(1,1-dimethylethyl)-4-[[5-(1,1-dimethylethyl)-4-hydroxy-2-methylphenyl]thio]-5-methylphenoxy]-acetic acid ethyl ester	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate 4-semialdehyde + NAD(P)H	Saccharomyces cerevisiae	essential step in amino acids L-methionine, L-threonine, and L-isoleucine biosynthesis	L-homoserine + NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate 4-semialdehyde + NAD(P)H	-	Saccharomyces cerevisiae	L-homoserine + NAD(P)+	-	?
L-aspartate 4-semialdehyde + NAD(P)H	essential step in amino acids L-methionine, L-threonine, and L-isoleucine biosynthesis	Saccharomyces cerevisiae	L-homoserine + NAD(P)+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Saccharomyces cerevisiae
NADPH	-	Saccharomyces cerevisiae