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Literature summary for 1.1.1.3 extracted from

  • Sainis, J.K.; Mayne, R.G.; Wallsgrove, R.M.; Lea, P.J.; Miflin, B.J.
    Localisation and characterisation of homoserine dehydrogenase isolated from barley and pea leaves (1981), Planta, 152, 491-496.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme Hordeum vulgare
L-cysteine slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme Pisum sativum
L-threonine
-
Hordeum vulgare
L-threonine
-
Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
NADPH isozyme II Pisum sativum
0.04
-
aspartate 4-semialdehyde isozyme II Pisum sativum
0.046
-
NADPH isozyme I Pisum sativum
0.066
-
L-aspartate 4-semialdehyde isozyme I Pisum sativum
0.1
-
L-aspartate 4-semialdehyde isozyme I Hordeum vulgare
0.36 0.4 L-aspartate 4-semialdehyde isozyme II Hordeum vulgare
0.46
-
NADH isozyme II Pisum sativum
0.46
-
NADH isoenzyme II, at pH 7.5, temperature not specified in the publication Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme I Hordeum vulgare 9507
-
chloroplast isozyme I Pisum sativum 9507
-
cytoplasm isozyme II Hordeum vulgare 5737
-
cytoplasm isozyme II Pisum sativum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration, isozyme II, cytoplasm Pisum sativum
69000
-
gel filtration, isozyme II, cytoplasm Hordeum vulgare
168000 174000 gel filtration, isozyme I, chloroplasts Hordeum vulgare
168000 174000 gel filtration, isozyme I, chloroplasts Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate 4-semialdehyde + NAD(P)H Hordeum vulgare third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine L-homoserine + NAD(P)+
-
r
L-aspartate 4-semialdehyde + NAD(P)H Pisum sativum third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine L-homoserine + NAD(P)+
-
r

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
barley
-
Pisum sativum
-
pea
-

Purification (Commentary)

Purification (Comment) Organism
separation of the two isozymes using Blue-Sepharose chromatography Pisum sativum
separation of the two isozymes using Matrex Gel Red A affinity chromatography Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Hordeum vulgare
-
leaf
-
Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + NAD(P)H
-
Hordeum vulgare L-homoserine + NAD(P)+
-
r
L-aspartate 4-semialdehyde + NAD(P)H
-
Pisum sativum L-homoserine + NAD(P)+
-
r
L-aspartate 4-semialdehyde + NAD(P)H third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine Hordeum vulgare L-homoserine + NAD(P)+
-
r
L-aspartate 4-semialdehyde + NAD(P)H third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine Pisum sativum L-homoserine + NAD(P)+
-
r
L-aspartate 4-semialdehyde + NADH + H+
-
Pisum sativum L-homoserine + NAD+
-
r
L-homoserine + NAD(P)+
-
Hordeum vulgare L-aspartate 4-semialdehyde + NAD(P)H
-
r
L-homoserine + NAD(P)+
-
Pisum sativum L-aspartate 4-semialdehyde + NAD(P)H
-
r

Cofactor

Cofactor Comment Organism Structure
NADH
-
Hordeum vulgare
NADH
-
Pisum sativum
NADPH preferred Hordeum vulgare
NADPH preferred Pisum sativum