Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme | Hordeum vulgare | |
L-cysteine | slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme | Pisum sativum | |
L-threonine | - |
Hordeum vulgare | |
L-threonine | - |
Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
NADPH | isozyme II | Pisum sativum | |
0.04 | - |
aspartate 4-semialdehyde | isozyme II | Pisum sativum | |
0.046 | - |
NADPH | isozyme I | Pisum sativum | |
0.066 | - |
L-aspartate 4-semialdehyde | isozyme I | Pisum sativum | |
0.1 | - |
L-aspartate 4-semialdehyde | isozyme I | Hordeum vulgare | |
0.36 | 0.4 | L-aspartate 4-semialdehyde | isozyme II | Hordeum vulgare | |
0.46 | - |
NADH | isozyme II | Pisum sativum | |
0.46 | - |
NADH | isoenzyme II, at pH 7.5, temperature not specified in the publication | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme I | Hordeum vulgare | 9507 | - |
chloroplast | isozyme I | Pisum sativum | 9507 | - |
cytoplasm | isozyme II | Hordeum vulgare | 5737 | - |
cytoplasm | isozyme II | Pisum sativum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration, isozyme II, cytoplasm | Pisum sativum |
69000 | - |
gel filtration, isozyme II, cytoplasm | Hordeum vulgare |
168000 | 174000 | gel filtration, isozyme I, chloroplasts | Hordeum vulgare |
168000 | 174000 | gel filtration, isozyme I, chloroplasts | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + NAD(P)H | Hordeum vulgare | third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine | L-homoserine + NAD(P)+ | - |
r | |
L-aspartate 4-semialdehyde + NAD(P)H | Pisum sativum | third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine | L-homoserine + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Pisum sativum | - |
pea | - |
Purification (Comment) | Organism |
---|---|
separation of the two isozymes using Blue-Sepharose chromatography | Pisum sativum |
separation of the two isozymes using Matrex Gel Red A affinity chromatography | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hordeum vulgare | - |
leaf | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + NAD(P)H | - |
Hordeum vulgare | L-homoserine + NAD(P)+ | - |
r | |
L-aspartate 4-semialdehyde + NAD(P)H | - |
Pisum sativum | L-homoserine + NAD(P)+ | - |
r | |
L-aspartate 4-semialdehyde + NAD(P)H | third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine | Hordeum vulgare | L-homoserine + NAD(P)+ | - |
r | |
L-aspartate 4-semialdehyde + NAD(P)H | third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine | Pisum sativum | L-homoserine + NAD(P)+ | - |
r | |
L-aspartate 4-semialdehyde + NADH + H+ | - |
Pisum sativum | L-homoserine + NAD+ | - |
r | |
L-homoserine + NAD(P)+ | - |
Hordeum vulgare | L-aspartate 4-semialdehyde + NAD(P)H | - |
r | |
L-homoserine + NAD(P)+ | - |
Pisum sativum | L-aspartate 4-semialdehyde + NAD(P)H | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Hordeum vulgare | |
NADH | - |
Pisum sativum | |
NADPH | preferred | Hordeum vulgare | |
NADPH | preferred | Pisum sativum |