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Literature summary for 1.1.1.292 extracted from

  • Kuehn, A.; Yu, S.; Giffhorn, F.
    Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis (2006), Appl. Environ. Microbiol., 72, 1248-1257.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.292

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Ensifer adhaerens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 NADPH pH 6.5, 30°C Ensifer adhaerens
8.4
-
 1,5-Anhydro-D-fructose pH 6.5, 30°C Ensifer adhaerens
11
-
 D-glucosone pH 6.5, 30°C Ensifer adhaerens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35100
-
 1 * 35100, MALDI-TOF-MS Ensifer adhaerens
38200
-
 gel filtration Ensifer adhaerens
40000
-
 1 * 40000, SDS-PAGE Ensifer adhaerens

Organism

Organism UniProt Comment Textmining
Ensifer adhaerens Q2I8V6 i.e. Ensifer adhaerens
-
Ensifer adhaerens S-30.7.5 Q2I8V6 i.e. Ensifer adhaerens
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme Ensifer adhaerens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
288.9
-
 native enzyme Ensifer adhaerens
484
-
 recombinant enzyme expressed in Escherichia coli Ensifer adhaerens

Storage Stability

Storage Stability Organism
-20°C or 0°C, 50 days, 50% loss of activity Ensifer adhaerens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADPH + H+
-
 Ensifer adhaerens 1,5-anhydro-D-mannitol + NADP+
-
 r
1,5-anhydro-D-fructose + NADPH + H+
-
 Ensifer adhaerens S-30.7.5 1,5-anhydro-D-mannitol + NADP+
-
 r
1,5-anhydro-D-glucitol + NADP+ 23% of the activity with 1,5-anhydro-D-mannitol Ensifer adhaerens ?
-
 ?
1,5-anhydro-D-glucitol + NADP+ 23% of the activity with 1,5-anhydro-D-mannitol Ensifer adhaerens S-30.7.5 ?
-
 ?
6-deoxy-D-glucosone + NADPH + H+ 22% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens 6-deoxy-D-mannose + NADP+
-
 ?
6-deoxy-D-glucosone + NADPH + H+ 22% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens S-30.7.5 6-deoxy-D-mannose + NADP+
-
 ?
D-allosone + NADPH + H+ 10% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens D-altrose + NADP+
-
 ?
D-allosone + NADPH + H+ 10% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens S-30.7.5 D-altrose + NADP+
-
 ?
D-glucosone + NADPH + H+ 22% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens D-mannose + NADP+
-
 ?
D-glucosone + NADPH + H+ 22% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens S-30.7.5 D-mannose + NADP+
-
 ?
D-xylosone + NADPH + H+ 17% of the activity with 1,5-anhydro-D-fructose Ensifer adhaerens D-lyxose + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Ensifer adhaerens
monomer 1 * 35100, MALDI-TOF-MS Ensifer adhaerens

Synonyms

Synonyms Comment Organism
1,5-anhydro-D-fructose reductase (ambiguous)
-
 Ensifer adhaerens
AFR
-
 Ensifer adhaerens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
63.2
-
 D-glucosone pH 6.5, 30°C Ensifer adhaerens
286.7
-
 1,5-Anhydro-D-fructose pH 6.5, 30°C Ensifer adhaerens
1300
-
 1,5-Anhydro-D-fructose pH 6.5, 30°C Ensifer adhaerens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Ensifer adhaerens

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.8 about 50% of maximal activity at pH 5.5 and at pH 8.8 Ensifer adhaerens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ensifer adhaerens
NADPH inactive towards NADH Ensifer adhaerens

pI Value

Organism Comment pI Value Maximum pI Value
Ensifer adhaerens isoelectric focusing
-
 4.3