Literature summary for 1.1.1.289 extracted from

Cai, P.; An, M.; Xu, L.; Xu, S.; Hao, N.; Li, Y.; Guo, K.; Yan, M.
Development of a substrate-coupled biocatalytic process driven by an NADPH-dependent sorbose reductase from Candida albicans for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate (2012), Biotechnol. Lett., 34, 2223-2227.

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Application

Application	Comment	Organism
synthesis	usage of a substrate-coupled biocatalytic process driven by an NADPH-dependent sorbose reductase from Candida albicans for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate	Candida albicans

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain Rosseta (DE3)	Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-sorbitol + NADP+	Candida albicans	-	L-sorbose + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannitol + NADP+	-	Candida albicans	D-mannose + NADPH + H+	-	r
D-sorbitol + NADP+	-	Candida albicans	L-sorbose + NADPH + H+	-	r
D-xylitol + NADP+	-	Candida albicans	D-xylose + NADPH + H+	-	r
additional information	development of a substrate-coupled biocatalytic process driven by NADPH-dependent sorbose reductase for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, overview. Ethyl 4-chloro-3-oxobutanoate is reduced to (S)-4-chloro-3-hydroxybutanoate, while NADPH is regenerated by the enzyme via oxidation of sorbitol, mannitol, or xylitol in crude extract of recombinant Escherichia coli cells, overview	Candida albicans	?	-	?

Synonyms

Synonyms	Comment	Organism
NADPH-dependent sorbose reductase	-	Candida albicans
SOU1	-	Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.2	-	assay at	Candida albicans

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	dependent on	Candida albicans

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Release 2023.1 (January 2023)