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Literature summary for 1.1.1.286 extracted from

  • Miyazaki, K.
    Identification of a novel trifunctional homoisocitrate dehydrogenase and modulation of the broad substrate specificity through site-directed mutagenesis (2005), Biochem. Biophys. Res. Commun., 336, 596-602.
    View publication on PubMed
Search for more literature for 1.1.1.286

Protein Variants

Protein Variants Comment Organism
additional information deletion of A80, inability of mutant to use 3-isopropylmalate, isocitrate is 4fold preferred over homoisocitrate Deinococcus radiodurans
R85V increased ability to use 3-isopropylmalate Deinococcus radiodurans
R87T uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate Deinococcus radiodurans
R87V uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate Deinococcus radiodurans

Inhibitors

Inhibitors Comment Organism Structure
K+ absolute requirement, inhibitory above 0.4 M Deinococcus radiodurans
Mn2+ inhibitory above 1 mM Deinococcus radiodurans
NH4+ may substitute for K+, inhibitory above 0.2 M Deinococcus radiodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.193
-
 NAD+ 30°C, pH 7.8 Deinococcus radiodurans
0.211
-
 homoisocitrate 30°C, pH 7.8, wild-type Deinococcus radiodurans
0.291
-
 isocitrate 30°C, pH 7.8, wild-type Deinococcus radiodurans
0.465
-
 isocitrate 30°C, pH 7.8, A80 deletion mutant Deinococcus radiodurans
0.819
-
 homoisocitrate 30°C, pH 7.8, A80 deletion mutant Deinococcus radiodurans
1
-
 homoisocitrate 30°C, pH 7.8, mutant R87V Deinococcus radiodurans
1.33
-
 3-isopropylmalate 30°C, pH 7.8, wild-type Deinococcus radiodurans
1.5
-
 homoisocitrate 30°C, pH 7.8, mutant R87T Deinococcus radiodurans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ absolute requirement, inhibitory above 0.4 M Deinococcus radiodurans
Mg2+ divalent cation required, Mg2+ is preferred over Mn2+ Deinococcus radiodurans
Mn2+ divalent cation required, Mg2+ is preferred over Mn2+, inhibitory above 1 mM Deinococcus radiodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35727
-
 4 * 35751, mass spectrometry, 4 * 35727, calculated Deinococcus radiodurans
35751
-
 4 * 35751, mass spectrometry, 4 * 35727, calculated Deinococcus radiodurans
154000
-
 gel filtration Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-isopropylmalate + NAD+ at 0.1% of the rate with homoisocitrate Deinococcus radiodurans ?
-
 ?
homoisocitrate + NAD+ 1.5fold preferred over isocitrate Deinococcus radiodurans ? + NADH
-
 ?
isocitrate + NAD+
-
 Deinococcus radiodurans 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NADP+ NAD+ is preferred over NADP+ Deinococcus radiodurans 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
additional information no substrate: NADP+ Deinococcus radiodurans ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 35751, mass spectrometry, 4 * 35727, calculated Deinococcus radiodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.371
-
 3-isopropylmalate 30°C, pH 7.8, wild-type Deinococcus radiodurans
5.46
-
 homoisocitrate 30°C, pH 7.8, A80 deletion mutant Deinococcus radiodurans
10.9
-
 isocitrate 30°C, pH 7.8, A80 deletion mutant Deinococcus radiodurans
20.8
-
 homoisocitrate 30°C, pH 7.8, mutant R87V Deinococcus radiodurans
27.8
-
 homoisocitrate 30°C, pH 7.8, mutant R87T Deinococcus radiodurans
30.3
-
 NAD+ 30°C, pH 7.8 Deinococcus radiodurans
42.8
-
 isocitrate 30°C, pH 7.8, wild-type Deinococcus radiodurans
46.2
-
 homoisocitrate 30°C, pH 7.8, wild-type Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Deinococcus radiodurans