Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | dependent on | Taiwanofungus camphoratus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in HEK 293T cells, transfectioon via a v-raf/v-myc oncogene-carrying retrovirus, improvement by codon optimization. The Tc-GFD protein expressed in pcGFD-O transfected 293T cells provides protection to these cells against apoptosis under GSNO induced oxidative stress, overview | Taiwanofungus camphoratus |
Protein Variants | Comment | Organism |
---|---|---|
C47S | site-directed mutagenesis, active site mutant | Taiwanofungus camphoratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-(hydroxymethyl)glutathione + NAD+ | Taiwanofungus camphoratus | - |
S-formylglutathione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taiwanofungus camphoratus | D2CFJ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-(hydroxymethyl)glutathione + NAD+ | - |
Taiwanofungus camphoratus | S-formylglutathione + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GFD | - |
Taiwanofungus camphoratus |
Glutathione-dependent formaldehyde dehydrogenase | - |
Taiwanofungus camphoratus |
Tc-GFD | formerly named Ac-GFD | Taiwanofungus camphoratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Taiwanofungus camphoratus |
General Information | Comment | Organism |
---|---|---|
physiological function | glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. Formaldehyde detoxification involves the conversion of formaldehyde to formate, which consists of three reactions: The first reaction is a spontaneous condensation reaction between formaldehyde and glutathione producing S-hydroxymethylglutathione (HMGSH). The second reaction, catalyzed by enzyme GFD, is the oxidation of HMGSH to form S-formylglutathione. In the third reaction, catalyzed by S-formylglutathione hydrolase (FGH), formate is formed and glutathione regenerated | Taiwanofungus camphoratus |