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Literature summary for 1.1.1.284 extracted from

  • Tavares, A.F.; Nobre, L.S.; Melo, A.M.; Saraiva, L.M.
    A novel nitroreductase of Staphylococcus aureus with S-nitrosoglutathione reductase activity (2009), J. Bacteriol., 191, 3403-3406.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.284

Cloned(Commentary)

Cloned (Comment) Organism
wild-type cloned into pET28a(+), yielding pET28NtrA, and transformed in Escherichia coli BL21-Gold(DE3). DELTAntrA mutant (LMSA833) ligated to pSP64D-E (pSP64-SA0833) and integrated into Staphylococcus aureus RN4220 Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1806
-
 S-nitrosoglutathione at pH 7.6 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
 NCTC 8325
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity for NO, S-nitrosocysteine, or S-nitrosohomocysteine Staphylococcus aureus ?
-
 ?
S-nitrosoglutathione + NAD(P)H + H+ NADPH-dependent activity is higher than NADH-dependent activity Staphylococcus aureus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
nitroreductase
-
 Staphylococcus aureus
NTRA
-
 Staphylococcus aureus
S-nitrosoglutathione reductase
-
 Staphylococcus aureus
SA0UHSC_00833
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 S-nitrosoglutathione at pH 7.6 Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Staphylococcus aureus
NADPH NADPH-dependent activity is higher than NADH-dependent activity Staphylococcus aureus

Expression

Organism Comment Expression
Staphylococcus aureus S-nitrosoglutathione significantly upregulates the SA0UHSC_00833 gene up

General Information

General Information Comment Organism
physiological function NtrA is a bifunctional enzyme that exhibits nitroreductase and S-nitrosoglutathione reductase activity. Even though the presence of NtrA contributes to the activation of nitrofurans, in the absence of these antibiotics NtrA acts as a defense system that allows the decomposition of endogenously formed S-nitrosoglutathione, which occurs when Staphylococcus aureus is exposed to nitrosative stress, thus avoiding the harmful effects caused by transnitrosylation reactions. Inactivation of the putative nitroreductase SA0UHSC_00833 increases the sensitivity to S-nitrosoglutathione and augments its resistance to nitrofurans. Expression of NtrA from the pMK4 plasmid restores the wild-type phenotype Staphylococcus aureus