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Literature summary for 1.1.1.284 extracted from

  • Nikitina, O.; Shleev, S.; Gayda, G.; Demkiv, O.; Gonchar, M.; Gorton, L.; Csoeregi, E.; Nistor, M.
    Bi-enzyme biosensor based on NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay (2007), Sens. Actuators B Chem., B125, 1-9.
No PubMed abstract available
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Application

Application Comment Organism
analysis usage of the enzyme in a bi-enzyme biosensor based on NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase activity and diaphorase activity for a formaldehyde assay, overview Ogataea angusta

Protein Variants

Protein Variants Comment Organism
additional information construction of a bi-enzyme biosensor based on NAD+- and glutathione-dependent recombinant genetically-engineered methylotrophic yeast formaldehyde dehydrogenase activity and from Bacillus stearothermophilus diaphorase activity as bio-recognition elements for a formaldehyde assay, overview, the sensor architecture comprises a first layer containing diaphorase cross-linked with an osmium complex-modified redox polymer (poly(vinylpyridine)-[osmium-(N,N'-methylated-2,2'-biimidalzole)3]3+/2+ complex) and a second layer formed by additional cross-linking of FDH with poly(ethylene glycol)(400)diglycidyl ether on the top, optimization, overview Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + glutathione + NAD+ Ogataea angusta the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ Ogataea angusta Tf 11-6 the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione S-formylglutathione + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
 genetically-engineered methylotrophic yeast
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+ the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta S-formylglutathione + NADH + H+
-
 ?
formaldehyde + glutathione + NAD+ the true substrate is S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Ogataea angusta Tf 11-6 S-formylglutathione + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Ogataea angusta