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Literature summary for 1.1.1.284 extracted from
- S-Nitrosogluthathione reductase activity of amphioxus ADH3: insights into the nitric oxide metabolism (2006), Int. J. Biol. Sci., 2, 117-124.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Branchiostoma floridae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
S-hydroxymethylglutathione | in 0.1 M sodium diphosphate at pH 8.0 | Branchiostoma floridae |  |
| 0.008 | - |
NAD+ | in 0.1 M sodium diphosphate at pH 7.5 | Branchiostoma floridae | |
| 0.045 | - |
NADH | in 0.1 M sodium diphosphate at pH 7.5 | Branchiostoma floridae | |
| 0.056 | - |
S-nitrosoglutathione | in 0.1 M sodium diphosphate at pH 8.0 | Branchiostoma floridae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Branchiostoma floridae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity resin chromatography | Branchiostoma floridae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | - |
Branchiostoma floridae | - |
| additional information | dorsal region of the club-shaped gland | Branchiostoma floridae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-hydroxymethylglutathione + NAD+ | - |
Branchiostoma floridae | S-formylglutathione + NADH + H+ | - |
? | |
| S-nitrosoglutathione + NADH | - |
Branchiostoma floridae | GSH + NAD+ + NO | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH3 | - |
Branchiostoma floridae |
| alcohol dehydrogenase class III | - |
Branchiostoma floridae |
| FALDH | - |
Branchiostoma floridae |
| Glutathione-dependent formaldehyde dehydrogenase | - |
Branchiostoma floridae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
S-hydroxymethylglutathione | - |
Branchiostoma floridae | |
| 16.7 | - |
S-nitrosoglutathione | - |
Branchiostoma floridae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Branchiostoma floridae | |
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Release 2023.1 (January 2023)
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