Protein Variants | Comment | Organism |
---|---|---|
D267E | kinetic properties identical to that of wild-type enzyme | Saccharomyces cerevisiae |
D267E/T269I | Km-value for S-hydroxymethylglutathione is about 11fold lower than that of the wild-type enzyme, turnover-number for S-hydroxymethylglutathione is about 8fold lower than that of wild-type enzyme | Saccharomyces cerevisiae |
D269I | highly unstable enzyme, kinetic properties identical to that of wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
S-hydroxymethyl glutathione | mutant D267E/T269I | Saccharomyces cerevisiae | |
0.03 | - |
S-hydroxymethylglutathione | wild-type enzyme | Saccharomyces cerevisiae | |
0.045 | - |
NAD+ | wilde-type enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
non-denaturing PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | Saccharomyces cerevisiae | enzyme is not essential but enhances the resistance against formaldehyde | S-formylglutathione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione | Saccharomyces cerevisiae | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | enzyme is not essential but enhances the resistance against formaldehyde | Saccharomyces cerevisiae | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + S-hydroxymethyl glutathione + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.75 | - |
S-hydroxymethyl glutathione | mutant D267E/T269I | Saccharomyces cerevisiae | |
51.7 | - |
S-hydroxymethylglutathione | wild-type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |