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Literature summary for 1.1.1.284 extracted from

  • Kato, N.; Miyawaki, N.; Sakazawa, C.
    Formaldehyde dehydrogenase from formaldehyde-resistant Debaryomyces vanriji FT-1 and Pseudomonas putida F61 (1983), Agric. Biol. Chem., 47, 415-416.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
NAD+
-
Pseudomonas putida
0.31
-
NAD+
-
Schwanniomyces vanrijiae
0.39
-
formaldehyde
-
Pseudomonas putida
1.14
-
formaldehyde
-
Schwanniomyces vanrijiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Schwanniomyces vanrijiae
42000
-
2 * 42000, SDS-PAGE Pseudomonas putida
72000
-
gel filtration Pseudomonas putida
72000
-
gel filtration Schwanniomyces vanrijiae

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida F61
-
-
-
Schwanniomyces vanrijiae
-
-
-
Schwanniomyces vanrijiae FT-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida
-
Schwanniomyces vanrijiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
184
-
-
Schwanniomyces vanrijiae
185
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month Pseudomonas putida
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month Schwanniomyces vanrijiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
Pseudomonas putida S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Schwanniomyces vanrijiae S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Pseudomonas putida F61 S-formylglutathione + NADH + H+
-
?
formaldehyde + glutathione + NAD+
-
Schwanniomyces vanrijiae FT-1 S-formylglutathione + NADH + H+
-
?
methylglyoxal + glutathione + NAD+
-
Pseudomonas putida S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+
-
Schwanniomyces vanrijiae S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+
-
Pseudomonas putida F61 S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+
-
Schwanniomyces vanrijiae FT-1 S-pyruvylglutathione + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Schwanniomyces vanrijiae
dimer 2 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Schwanniomyces vanrijiae
45
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
10 min, stable Schwanniomyces vanrijiae
40
-
10 min, stable Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Schwanniomyces vanrijiae
9
-
-
Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 4°C, 3 d, stable Pseudomonas putida
6.5 8.5 4°C, 3 d, stable Schwanniomyces vanrijiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NAD+
-
Schwanniomyces vanrijiae