Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.283 extracted from

  • Liu, S.; Skory, C.; Qureshi, N.
    Ethanol tolerance assessment in recombinant E. coli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929 (2020), World J. Microbiol. Biotechnol., 36, 179 .
    View publication on PubMed
Search for more literature for 1.1.1.283

Cloned(Commentary)

Cloned (Comment) Organism
gene Lbuc_0522, recombinant overexpression in Escherichia coli strain BL21(DE3)pLysS Lentilactobacillus buchneri

Protein Variants

Protein Variants Comment Organism
additional information the recombinant enzyme overexpressed in Escherichia coli strain BL21(DE3)pLysS is tolerant against 4% v/v ethanol in the growth medium Lentilactobacillus buchneri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactaldehyde + NADP+ Lentilactobacillus buchneri
-
 methylglyoxal + NADPH + H+
-
 ?
(S)-lactaldehyde + NADP+ Lentilactobacillus buchneri NRRL B-30929
-
 methylglyoxal + NADPH + H+
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol the recombinant enzyme overexpressed in Escherichia coli strain BL21(DE3)pLysS is tolerant against 4% v/v ethanol in the growth medium Lentilactobacillus buchneri

Organism

Organism UniProt Comment Textmining
Lentilactobacillus buchneri
-
-
-
Lentilactobacillus buchneri NRRL B-30929
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactaldehyde + NADP+
-
 Lentilactobacillus buchneri methylglyoxal + NADPH + H+
-
 ?
(S)-lactaldehyde + NADP+
-
 Lentilactobacillus buchneri NRRL B-30929 methylglyoxal + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
Lbuc_0522
-
 Lentilactobacillus buchneri
MG reductase
-
 Lentilactobacillus buchneri
NADPH-dependent methylglyoxal reductase
-
 Lentilactobacillus buchneri

General Information

General Information Comment Organism
evolution MG reductases are highly conserved Lentilactobacillus buchneri
physiological function the enzyme is involved in detoxification of methylglyoxal (MG), a cytotoxic by-product of glycolysis that is identified as a potential glycolytic inhibitor, which inhibits the growth of glucose-fermenting yeast cells by promoting degradation of the glucose sensors. The MG reductase gene has been reported to play a critical role in maintaining redox balance during ethanol fermentation in yeasts Lentilactobacillus buchneri