Cloned (Comment) | Organism |
---|---|
gene rmd, constitutive recombinant overexpression in Spodoptera frugiperda Sf9 cells or in Trichoplusia ni High Five cells under the transcriptional control of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) immediate early 1 (ie1) promoter and via baculovirus transfection. Rmd to blocks the production of GDP-L-fucose and glycoprotein fucosylation because GDP-L-fucose is required as the donor substrate for this process | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GDP-L-fucose biosynthesis in eukaryotic cells is reduced by expression of bacterial Rmd, which converts GDP-4-dehydro-6-deoxy-D-mannose to GDP-D-rhamnose, assessing a vector-based glycoengineering strategy for blocking glycoprotein fucosylation in insect cells, overview. N-glycan profiling by MALDI-TOF mass spectrometry | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+ | Pseudomonas aeruginosa | - |
GDP-D-rhamnose + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTB6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+ | - |
Pseudomonas aeruginosa | GDP-D-rhamnose + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDP-6-deoxy-D-mannose reductase | - |
Pseudomonas aeruginosa |
RMD | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme consumes the immediate precursor to GDP-L-fucose to produce GDP-D-rhamnose, which might be a dead-end product in recombinant insect cells | Pseudomonas aeruginosa |