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Literature summary for 1.1.1.281 extracted from

  • Mäki, M.; Järvinen, N.; Räbinä, J.; Roos, C.; Maaheimo, H.; Mattila, P.; Renkonen, R.
    Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose (2002), Eur. J. Biochem., 269, 593-601.
    View publication on PubMed

Application

Application Comment Organism
synthesis generating GDP-rhamnose for in vitro rhamnosylation of glycoproteins and glycopeptides Pseudomonas aeruginosa
synthesis the functional expression of the Pseudomonas aeruginosa enzyme in Saccharomyces cerevisiae will provide a tool for generating GDP-rhamnose for in vitro rhamnosylation of glycoprotein and glycopeptides Pseudomonas aeruginosa
synthesis generation of GDP-rhamnose by coexpression of enzyme with GDP-mannose-4,6-dehydratase in Saccharomyces cerevisiae Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa
34000
-
x * 34000, recombinant FLAG-tagged enzyme Pseudomonas aeruginosa
34000
-
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NADPH Pseudomonas aeruginosa
-
GDP-6-deoxy-D-mannose + NADP+
-
?
GDP-6-deoxy-D-mannose + NAD(P)+ Pseudomonas aeruginosa
-
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
-
expression in Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
-
Pseudomonas aeruginosa GDP-6-deoxy-D-mannose + NADP+
-
?
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
-
Pseudomonas aeruginosa GDP-alpha-D-rhamnose + NAD+
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
-
Pseudomonas aeruginosa GDP-alpha-D-rhamnose + NADP+
-
ir
GDP-6-deoxy-D-mannose + NAD(P)+
-
Pseudomonas aeruginosa GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
?

Subunits

Subunits Comment Organism
? x * 34000, recombinant FLAG-tagged enzyme Pseudomonas aeruginosa
? x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas aeruginosa
NADH
-
Pseudomonas aeruginosa
NADP+
-
Pseudomonas aeruginosa
NADPH
-
Pseudomonas aeruginosa