Literature summary for 1.1.1.28 extracted from

Kim, S.; Kim, Y.H.; Kim, K.J.
Cloning, expression, purification, crystallization and X-ray crystallographic analysis of D-lactate dehydrogenase from Lactobacillus jensenii (2014), Acta Crystallogr. Sect. F, 70, 1046-1048.

Application

Application	Comment	Organism
synthesis	the polymers of lactic acid are used as biodegradable bioplastics. Polylactic acid, biodegradable polyester polymer of lactic acid, is mainly produced through a bacterial fermentation process	Lactobacillus jensenii

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834	Lactobacillus jensenii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, mixing of 0.0015 ml of 35 mg/ml protein in 40 mM Tris-HCl, pH 8.0, and 1 mM DTT with 0.0015 ml of reservoir solution containing 28% w/v PEG 400, 100 mM TrisHCl, pH 9.0, 200 mM magnesium sulfate at 22°C, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution by single wavelength anomalous dispersion using a selenomethionine derivative	Lactobacillus jensenii

Crystallization (Comment)

Organism

purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, mixing of 0.0015 ml of 35 mg/ml protein in 40 mM Tris-HCl, pH 8.0, and 1 mM DTT with 0.0015 ml of reservoir solution containing 28% w/v PEG 400, 100 mM TrisHCl, pH 9.0, 200 mM magnesium sulfate at 22°C, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution by single wavelength anomalous dispersion using a selenomethionine derivative

Lactobacillus jensenii

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus jensenii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged selenomethionine-labeled enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration	Lactobacillus jensenii

Synonyms

Synonyms	Comment	Organism
Ljd-LDH	-	Lactobacillus jensenii

General Information

General Information	Comment	Organism
physiological function	thermostable D-lactate dehydrogenase is a key enzyme for the production of the D-form of lactic acid from pyruvate concomitant with the oxidation of NADH to NAD+	Lactobacillus jensenii