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Literature summary for 1.1.1.28 extracted from

  • Li, L.; Eom, H.J.; Park, J.M.; Seo, E.; Ahn, J.E.; Kim, T.J.; Kim, J.H.; Han, N.S.
    Characterization of the major dehydrogenase related to D-lactic acid synthesis in Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 (2012), Enzyme Microb. Technol., 51, 274-279.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lhdD or LEUM 1756, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Star Leuconostoc mesenteroides subsp. mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited by Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Na+, or urea Leuconostoc mesenteroides subsp. mesenteroides
SDS over 95% inhibition at 1 mM Leuconostoc mesenteroides subsp. mesenteroides
Zn2+ 70% inhibition at 1 mM Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
NADH pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
0.58
-
pyruvate pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
1.96
-
NAD+ pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
260
-
(R)-lactate pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Leuconostoc mesenteroides subsp. mesenteroides
-
pyruvate + NADH + H+
-
r
(R)-lactate + NAD+ Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides subsp. mesenteroides
-
gene ldhD or LEUM 1756
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
gene ldhD or LEUM 1756
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 1.82fold from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography Leuconostoc mesenteroides subsp. mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.78
-
purified recombinant enzyme, D-lactate oxidation, pH 11.0, 20°C Leuconostoc mesenteroides subsp. mesenteroides
4450
-
purified recombinant enzyme, NADH oxidation, pH 8.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
4990
-
purified recombinant enzyme, pyruvate reduction, pH 8.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides pyruvate + NADH + H+
-
r
(R)-lactate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 pyruvate + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer
-
Leuconostoc mesenteroides subsp. mesenteroides

Synonyms

Synonyms Comment Organism
D-(-)-lactate dehydrogenase
-
Leuconostoc mesenteroides subsp. mesenteroides
LDH
-
Leuconostoc mesenteroides subsp. mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
lactate oxidation reaction Leuconostoc mesenteroides subsp. mesenteroides
30
-
pyruvate reduction reaction Leuconostoc mesenteroides subsp. mesenteroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2280
-
(R)-lactate pH 11.0, 20°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides
29000
-
pyruvate pH 8.0, 30°C, recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
pyruvate reduction reaction Leuconostoc mesenteroides subsp. mesenteroides
11
-
lactate oxidation reaction Leuconostoc mesenteroides subsp. mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pyruvate reduction reaction, activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides
7 13 lactate oxidation reaction, activity range, profile overview Leuconostoc mesenteroides subsp. mesenteroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides
NADH
-
Leuconostoc mesenteroides subsp. mesenteroides