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Literature summary for 1.1.1.28 extracted from
- Characterization of D-lactate dehydrogenase from Pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid (2012), Biotechnol. Lett., 34, 907-911.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pediococcus acidilactici |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits 35% at 1 mM | Pediococcus acidilactici |  |
| Fe2+ | inhibits 25% at 1 mM | Pediococcus acidilactici | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
| 2.9 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates by 25% at 1 mM | Pediococcus acidilactici | |
| additional information | poor effects by 1 mM Ca2+, Zn2+, Mg2+, Ba2+, and Co2+ | Pediococcus acidilactici |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | Pediococcus acidilactici | - |
pyruvate + NADH + H+ | - |
r | |
| (R)-lactate + NAD+ | Pediococcus acidilactici DSM 20284 | - |
pyruvate + NADH + H+ | - |
r | |
| phenylpyruvate + NADH + H+ | Pediococcus acidilactici | - |
(R)-3-phenyllactate + NAD+ | - |
? | |
| phenylpyruvate + NADH + H+ | Pediococcus acidilactici DSM 20284 | - |
(R)-3-phenyllactate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pediococcus acidilactici | - |
- |
- |
| Pediococcus acidilactici DSM 20284 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pediococcus acidilactici |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140 | - |
purified recombinant enzyme, substrate phenylpyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
| 422 | - |
purified recombinant enzyme, substrate pyruvate, pH 5.5, 30°C | Pediococcus acidilactici |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + NAD+ | - |
Pediococcus acidilactici | pyruvate + NADH + H+ | - |
r | |
| (R)-lactate + NAD+ | - |
Pediococcus acidilactici DSM 20284 | pyruvate + NADH + H+ | - |
r | |
| phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici | (R)-3-phenyllactate + NAD+ | - |
? | |
| phenylpyruvate + NADH + H+ | - |
Pediococcus acidilactici DSM 20284 | (R)-3-phenyllactate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Pediococcus acidilactici |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-LDH | - |
Pediococcus acidilactici |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pediococcus acidilactici |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, profile, overview | Pediococcus acidilactici |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | the enzyme is significantly stable below 30°C, but the activity decreases significantly above 40°C | Pediococcus acidilactici |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 305 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Pediococcus acidilactici |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | activity range, profile, overview | Pediococcus acidilactici |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pediococcus acidilactici | |
| NADH | - |
Pediococcus acidilactici | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 105 | - |
phenylpyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
| 3157 | - |
pyruvate | recombinant enzyme, pH 5.5, 30°C | Pediococcus acidilactici | |
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