Cloned (Comment) | Organism |
---|---|
gene APE_0487, expression in Escherichia coli strain Rosetta-gami 2 (DE3) | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 15 mg/ml protein in 10 mM potassium phosphate, pH 7.0, with 0.002 ml of reservoir solution containing 14.4% PEG 8000, 80 mM cacodylate pH 6.5, 160 mM calcium acetate and 20% glycerol, and equilibration against 0.1 ml of reservoir solution, 25°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
1 * 59000, recombinant enzyme, SDS-PAGE | Aeropyrum pernix |
61000 | - |
recombinant enzyme, gel filtration | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Aeropyrum pernix | - |
pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | Aeropyrum pernix DSM 11879 | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YEU4 | gene APE_0487; gene APE_0487 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Aeropyrum pernix | pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | - |
Aeropyrum pernix DSM 11879 | pyruvate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, recombinant enzyme, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
DLDH | - |
Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aeropyrum pernix | |
NADH | - |
Aeropyrum pernix |