Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | 0.05 mM, about 40% residual activity | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
D-lactate | pH 8.0, 30°C | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Rhodopseudomonas palustris |
235000 | - |
gel filtration | Rhodopseudomonas palustris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
No. 7, grown photoanaerobically on lactate | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.5 | - |
pH 8.0, 30°C | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + cytochrome c2ox | - |
Rhodopseudomonas palustris | pyruvate + cytochrome c2red | - |
? | |
D-lactate + NAD+ | - |
Rhodopseudomonas palustris | pyruvate + NADH | - |
? | |
DL-2-hydroxybutyrate + NAD+ | about 44% of activity against D-lactate | Rhodopseudomonas palustris | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 57000, SDS-PAGE | Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable | Rhodopseudomonas palustris |
60 | - |
above, rapid decrease of activity | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodopseudomonas palustris | |
NAD+ | - |
Rhodopseudomonas palustris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
oxalate | pH 8.0, 30°C | Rhodopseudomonas palustris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodopseudomonas palustris | isoelectric focusing | - |
5 |