Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline 5-sulfonic acid | - |
Saccharomyces cerevisiae | |
HgCl2 | - |
Saccharomyces cerevisiae | |
o-phenanthroline | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ethyl 3-oxohexanoate | - |
Saccharomyces cerevisiae | |
17 | - |
ethyl 3-oxobutanoate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
210000 | - |
x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
780000 | 800000 | gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.958 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl 3-oxobutanoate + NADPH | - |
Saccharomyces cerevisiae | ethyl (R)-3-hydroxybutanoate + NADP+ | - |
? | |
ethyl 3-oxohexanoate + NADPH | - |
Saccharomyces cerevisiae | ethyl (R)-3-hydroxyhexanoate + NADP+ | - |
? | |
additional information | specific for 3-oxo-esters, no reaction with 3-oxo-acids | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 210000 + x * 200000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
rapid decrease of activity above | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Saccharomyces cerevisiae |