Cloned (Comment) | Organism |
---|---|
gene CTATCC11996_22452, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gulonate + NADP+ | Comamonas testosteroni | - |
2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
2-dehydro-D-gulonate + NADP+ | Comamonas testosteroni ATCC 11996 | - |
2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
additional information | Comamonas testosteroni | the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview | ? | - |
? | |
additional information | Comamonas testosteroni ATCC 11996 | the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Comamonas testosteroni ATCC 11996 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gulonate + NADP+ | - |
Comamonas testosteroni | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
2-dehydro-D-gulonate + NADP+ | - |
Comamonas testosteroni ATCC 11996 | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
additional information | the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview | Comamonas testosteroni | ? | - |
? | |
additional information | the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview | Comamonas testosteroni ATCC 11996 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39400, about, sequence calculation, x * 41600, recombinant His-tagged enzyme, SDS-PAGE | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconic acid reductase | - |
Comamonas testosteroni |
2,5DKGR | - |
Comamonas testosteroni |
CTATCC11996_22452 | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Comamonas testosteroni |
Organism | Comment | Expression |
---|---|---|
Comamonas testosteroni | the enzyme expression is significantly induced by estrone, estradiol, and methyltestosterone, and slightly by progesterone, but not by testosterone | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the AKR superfamily, monomeric (alpha/beta) 8-barrel proteins which bind NAD(P)(H) to metabolize an array of substrates | Comamonas testosteroni |
malfunction | compared to the wild-type, the knockout mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone. Cell growth on ethanol, oestrone, estradiol, testosterone or methyltestosterone is reduced in the mutant strain compared to the wild-type | Comamonas testosteroni |
additional information | three consensus sequences of the AKR superfamily are found as GxxxxDxAxxY, LxxxGxxxPxxGxG and LxxxxxxxxxDxxxxH. GxxxxDxAxxY is the active site, LxxxGxxxPxxGxG is the cofactor-binding site for NAD(P)(H), and LxxxxxxxxxDxxxxH is required for supporting the 3D structure | Comamonas testosteroni |