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Literature summary for 1.1.1.274 extracted from

  • Yum, D.Y.; Lee, B.Y.; Pan, J.G.
    Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli (1999), Appl. Environ. Microbiol., 65, 3341-3346.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis enzyme may be useful for the synthesis of the ascorbate precursor 2-keto-L-gulonate Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of isoenzymes A and B Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 29000, isoenzymes A and B, SDS-PAGE Escherichia coli
30000
-
isoenzymes A and B, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P30863
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isoenzymes A and B, affinity chitin-binding tag Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH both isoenzymes A and B are specific for NADPH Escherichia coli 2-keto-L-gulonate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, isoenzymes A and B, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
isoenzyme B Escherichia coli
7.5
-
isoenzyme A Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli