Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C109A | lower Km than wild-type enzyme | Escherichia coli |
H179N | lower Km than wild-type enzyme | Escherichia coli |
K140R | higher Km than wild-type enzyme | Escherichia coli |
K140S | higher Km than wild-type enzyme | Escherichia coli |
R187A | lower Km than wild-type enzyme | Escherichia coli |
S107A | lower Km than wild-type enzyme | Escherichia coli |
Y136E | no enzymatic activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for various mutants | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | 36000 | SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
wild type enzyme is stable for several weeks in phosphate buffered saline at concentrations higher than 10 mg/ml, stability of mutants differ from wild type enzyme | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values for various mutants | Escherichia coli |